Development of anaemia in inflammatory diseases is cytokine-mediated. Specifically, the levels of tumour necrosis factor-alpha (TNF-alpha), produced by activated macrophages, are correlated with severity of disease and anaemia in infections and chronic disease. In African trypanosomiasis, anaemia...
Naessens, Jan
,
Kitani, H.
,
Nakamura, Y.
,
Yagi, Y.
,
Sekikawa, K.
,
Iraqi, F.A.
,
[TNF-alpha mediates the development of anaemia in a murine Trypanosoma brucei rhodesiense infection, but not the anaemia associated with a murine Trypanosoma congolense infection]
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TNF-alpha mediates the development of anaemia in a murine Trypanosoma brucei rhodesiense infection, but not the anaemia associated with a murine Trypanosoma congolense infection
A murine IgM monoclonal antibody (mAb), IL-A77, has been generated that recognises the bovine transferrin receptor (TfR) and will be a useful tool to measure the activation state of bovine lymphocytes and macrophages. The antigen is detected on immature erythroid cells and proliferating lymphocyt...
Naessens, Jan
,
Grab, D.J.
,
Fritsch, G.
,
[Characterisation of bovine transferrin receptor on normal activated and Theileria parva-transformed lymphocytes by a new monoclonal antibody]
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Characterisation of bovine transferrin receptor on normal activated and Theileria parva-transformed lymphocytes by a new monoclonal antibody
C57BL/6 mice deficient in one or two copies of the gene for tumor necrosis factor alpha (TNF-α) were more susceptible to Trypanosoma congolense infection than their resistant, wild-type counterparts. The number of TNF-α genes was correlated with the capacity to control parasitaemia and with survi...
Naessens, Jan
,
Kitani, H.
,
Momotani, E.
,
Sekikawa, K.
,
Nthale, J.M.
,
Iraqi, F.A.
,
[Susceptibility of TNF-α-deficient mice to Trypanosoma congolense is not due to a defective antibody response]
,
Susceptibility of TNF-α-deficient mice to Trypanosoma congolense is not due to a defective antibody response
C57BL/6 mice deficient in one or two copies of the gene for tumor necrosis factor alpha (TNF-alpha) were more susceptible to Trypanosoma congolense infection than their resistant, wild-type counterparts. The number of TNF-alpha genes was correlated with the capacity to control parasitaemia and ...
Naessens, Jan
,
Kitani, H.
,
Momotani, E.
,
Sekikawa, K.
,
Nthale, J.M.
,
Iraqi, F.A.
,
[Susceptibility of TNF-alpha-deficient mice to Trypanosoma congolense is not due to a defective antibody response]
,
Susceptibility of TNF-alpha-deficient mice to Trypanosoma congolense is not due to a defective antibody response
After the preliminary clustering of the whole workshop panel using flowcytometry data, we selected those clusters that were composed of mAbs that bound a broad range of cells. To obtain evidence that mAbs in each of these preliminary clusters detected the same antigen, they were tested for their ...
Naessens, Jan
,
Nthale, J.M.
,
Muiya, P.
,
[Biochemical analysis of preliminary clusters in the non-lineage panel]
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Biochemical analysis of preliminary clusters in the non-lineage panel
Contagious bovine pleuropneumonia (CBPP) caused by Mycoplasma mycoides subsp. mycoides small colony type (MmmSC) has been eradicated in the developed world, but it is still present in many countries of sub-Saharan Africa. After initially successful control measures in the 1960s it has been spread...
Jores, Joerg
,
Meens, J.
,
Buettner, F.F.R.
,
Linz, B.
,
Naessens, Jan
,
Gerlach, G.F.
,
[Analysis of the immunoproteome of Mycoplasma mycoides subsp. mycoides small colony type reveals immunogenic homologues to other known virulence traits in related Mycoplasma species]
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Analysis of the immunoproteome of Mycoplasma mycoides subsp. mycoides small colony type reveals immunogenic homologues to other known virulence traits in related Mycoplasma species
Trypanotolerance is the capacity of certain West-African, taurine breeds of cattle to remain productive and gain weight after trypanosome infection. Laboratory studies, comparing Trypanosoma congolense infections in trypanotolerant N'Dama cattle (Bos taurus) and in more susceptible Boran cattle (...
Naessens, Jan
,
[Bovine trypanotolerance: a natural ability to prevent severe anaemia and haemophagocytic syndrome?]
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Bovine trypanotolerance: a natural ability to prevent severe anaemia and haemophagocytic syndrome?
IgD, first demonstrated in humans, has been unequivocally shown to exist in primates and rodents. In addition to lgM a second unique membrane isotype, generally considered to be lgD, has been demonstrated in a number of other species, including dogs and chickens. Because of its assumed widespread...
From a lysate of radiolabeled Theileria parva-infected bovine lymphocytes monoclonal antibody (mAb) IL-A77 immunoprecipitated a molecule of Mr 190 000 under non-reducing conditions, which was shown to be a homodimer of a Mr 90 000 protein after reduction (Naessens et al., 1996). Purification of t...