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Three international workshops held over the past eight years have addressed the identification and nomenclature of leukocyte differentiation antigens in ruminants. This article explains the progress the workshops have made.


Naessens, JanHoward, C.J.Hopkins, J.[Nomenclature and characterization of leukocyte differentiation antigens in ruminants]Nomenclature and characterization of leukocyte differentiation antigens in ruminants
Naessens, JanNthale, J.[Section 6.21. Biochemical characterization of three non-lineage antigens defined by workshop antibodies]Section 6.21. Biochemical characterization of three non-lineage antigens defined by workshop antibodies

Conditions were established to obtain depletion of T lymphocyte subsets in lymphoid tissues of calves by injection of mouse monoclonal antibodies to T cell antigens. Adverse reactions were avoided by injecting small quantities of antibody until target cells had disappeared from blood. Two differe...


Naessens, JanScheerlinck, J.P.Buysscher, E.V. deKennedy, D.Sileghem, M.R.[Effective in vivo depletion of T cell subpopulations and loss of memory cells in cattle using mouse monoclonal antibodies]Effective in vivo depletion of T cell subpopulations and loss of memory cells in cattle using mouse monoclonal antibodies

In this report, we describe the cloning, sequencing, and expression of the bovine orthologue of CD26 (BoCD26). Several monoclonal antibodies specific for a molecule, activation molecule 3 (ACT3), aberrantly expressed on superantigen-stimulated bovine CD8+ lymphocytes, reacted with recombinant BoC...


Sang-Un LeeYoung-Ho ParkDavis, W.C.Hamilton, M. JoNaessens, JanBohach, G.A.[Molecular characterization of bovine CD26 upregulated by a staphylococcal superantigen]Molecular characterization of bovine CD26 upregulated by a staphylococcal superantigen
Howard, C.J.Morrison, W. IvanBrown, W.C.Naessens, JanSopp, P.[Demonstration of two allelic forms of the bovine T cell antigen Bo5 (CD5) and studies of their inheritance]Demonstration of two allelic forms of the bovine T cell antigen Bo5 (CD5) and studies of their inheritance

Serum Ig from Trypanosoma congolense-infected cattle were affinity-purified using immobilised trypanosome or non-trypanosome antigens (beta-galactosidase, cytochrome C and ferritin). The bound and unbound IgG and IgM fractions were collected and tested in ELISA for reactivity to each antigen. The...


Buza, J.Naessens, Jan[Trypanosome non-specific IgM antibodies detected in serum of Trypanosoma congolense-infected cattle are polyreactive]Trypanosome non-specific IgM antibodies detected in serum of Trypanosoma congolense-infected cattle are polyreactive
Martini, J.C. deMacHugh, Niall D.Naessens, JanTeale, A.J.[Differential in vitro and in vivo expression of MHC class II antigens in bovine lymphocytes infected by Theileria parva]Differential in vitro and in vivo expression of MHC class II antigens in bovine lymphocytes infected by Theileria parva

Mice infected with African trypanosomes produce exceptionally large amounts of serum IgM, a major part of which binds to non-trypanosome antigens such as trinitrophenol and single-strand DNA. In this paper, we describe that in cattle infected with Trypanosoma congolense and T. vivax, similar anti...


Buza, J.J.Sileghem, M.R.Gwakisa, P.S.Naessens, Jan[CD5+B lymphocytes are the main source of antibodies reactive with non-parasite antigens in Trypanosoma congolense infected cattle]CD5+B lymphocytes are the main source of antibodies reactive with non-parasite antigens in Trypanosoma congolense infected cattle
Baldwin, C.L.MacHugh, Niall D.Ellis, J.A.Naessens, JanNewson, J.Morrison, W. Ivan[Monoclonal antibodies which react with bovine T-lymphocyte antigens and induce blastogenesis: Tissue distribution and functional characteristics of the target antigens]Monoclonal antibodies which react with bovine T-lymphocyte antigens and induce blastogenesis: Tissue distribution and functional characteristics of the target antigens

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