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Three international workshops held over the past eight years have addressed the identification and nomenclature of leukocyte differentiation antigens in ruminants. This article explains the progress the workshops have made.


Naessens, JanHoward, C.J.Hopkins, J.[Nomenclature and characterization of leukocyte differentiation antigens in ruminants]Nomenclature and characterization of leukocyte differentiation antigens in ruminants

A murine IgM monoclonal antibody (mAb), IL-A77, has been generated that recognises the bovine transferrin receptor (TfR) and will be a useful tool to measure the activation state of bovine lymphocytes and macrophages. The antigen is detected on immature erythroid cells and proliferating lymphocyt...


Naessens, JanGrab, D.J.Fritsch, G.[Characterisation of bovine transferrin receptor on normal activated and Theileria parva-transformed lymphocytes by a new monoclonal antibody]Characterisation of bovine transferrin receptor on normal activated and Theileria parva-transformed lymphocytes by a new monoclonal antibody
Naessens, Jan[Characterisation of lymphocyte populations in African buffalo (Syncerus caffer) and water buck (Kobus defassa) with workshop monoclonal antibodies]Characterisation of lymphocyte populations in African buffalo (Syncerus caffer) and water buck (Kobus defassa) with workshop monoclonal antibodies

Conditions were established to obtain depletion of T lymphocyte subsets in lymphoid tissues of calves by injection of mouse monoclonal antibodies to T cell antigens. Adverse reactions were avoided by injecting small quantities of antibody until target cells had disappeared from blood. Two differe...


Naessens, JanScheerlinck, J.P.Buysscher, E.V. deKennedy, D.Sileghem, M.R.[Effective in vivo depletion of T cell subpopulations and loss of memory cells in cattle using mouse monoclonal antibodies]Effective in vivo depletion of T cell subpopulations and loss of memory cells in cattle using mouse monoclonal antibodies
Naessens, JanSileghem, M.R.MacHugh, Niall D.Park, Y.H.Davis, W.C.Toye, Philip G.[Selection of BoCD25 monoclonal antibodies by screening mouse L cells transfected with the bovine p55-interleukin-2 (IL-2) receptor gene]Selection of BoCD25 monoclonal antibodies by screening mouse L cells transfected with the bovine p55-interleukin-2 (IL-2) receptor gene
Fritsch, G.Nelson, R.T.Muiya, P.Naessens, JanBlack, S.J.[Characterization of bovine haemopoietic progenitor cells using monoclonal antibodies and fluorocytometry]Characterization of bovine haemopoietic progenitor cells using monoclonal antibodies and fluorocytometry

Alpha gamma T cells in ruminants can be subdivided in two or more subpopulations on the basis of the expression of surface antigen WC1, which can exist in different isoforms. In this study, 18 monoclonal antibodies (mAbs) submitted to the Third International Workshop that were predicted to react ...


Davis, W.C.Brown, W.C.Hamilton, M.J.Wyatt, C.R.Orden, J.A.Khalid, A.M.Naessens, Jan[Analysis of monoclonal antibodies specific for the gamma alpha TcR]Analysis of monoclonal antibodies specific for the gamma alpha TcR
Baldwin, C.L.MacHugh, Niall D.Ellis, J.A.Naessens, JanNewson, J.Morrison, W. Ivan[Monoclonal antibodies which react with bovine T-lymphocyte antigens and induce blastogenesis: Tissue distribution and functional characteristics of the target antigens]Monoclonal antibodies which react with bovine T-lymphocyte antigens and induce blastogenesis: Tissue distribution and functional characteristics of the target antigens
Baldwin, C.L.Teale, A.J.Naessens, JanGoddeeris, B.M.MacHugh, Niall D.Morrison, W. Ivan[Characterization of a subset of bovine T lymphocytes that express BoT4 by monoclonal antibodies and function: Similarity to lymphocytes defined by human T4 and murine L3T4]Characterization of a subset of bovine T lymphocytes that express BoT4 by monoclonal antibodies and function: Similarity to lymphocytes defined by human T4 and murine L3T4

Monoclonal antibodies potentially specific for antigens expressed or upregulated on activated leukocytes were selected for further analysis from the panel submitted to the third international workshop on ruminant leukocyte antigens. The kinetics of expression of these activation antigens or resti...


Davis, W.C.Naessens, JanBrown, W.C.Ellis, J.A.Hamilton, M.J.Canton, G.H.Barbosa, J.J.R.Ferens, W.Bohach, G.A.[Analysis of monoclonal antibodies reactive with molecules upregulated or expressed only on activated lymphocytes]Analysis of monoclonal antibodies reactive with molecules upregulated or expressed only on activated lymphocytes

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