Three international workshops held over the past eight years have addressed the identification and nomenclature of leukocyte differentiation antigens in ruminants. This article explains the progress the workshops have made.
Naessens, Jan
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Howard, C.J.
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Hopkins, J.
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[Nomenclature and characterization of leukocyte differentiation antigens in ruminants]
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Nomenclature and characterization of leukocyte differentiation antigens in ruminants
From a lysate of radiolabeled Theileria parva-infected bovine lymphocytes monoclonal antibody (mAb) IL-A77 immunoprecipitated a molecule of Mr 190 000 under non-reducing conditions, which was shown to be a homodimer of a Mr 90 000 protein after reduction (Naessens et al., 1996). Purification of t...
Alpha gamma T cells in ruminants can be subdivided in two or more subpopulations on the basis of the expression of surface antigen WC1, which can exist in different isoforms. In this study, 18 monoclonal antibodies (mAbs) submitted to the Third International Workshop that were predicted to react ...
Davis, W.C.
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Brown, W.C.
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Hamilton, M.J.
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Wyatt, C.R.
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Orden, J.A.
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Khalid, A.M.
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Naessens, Jan
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[Analysis of monoclonal antibodies specific for the gamma alpha TcR]
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Analysis of monoclonal antibodies specific for the gamma alpha TcR
Monoclonal antibodies potentially specific for antigens expressed or upregulated on activated leukocytes were selected for further analysis from the panel submitted to the third international workshop on ruminant leukocyte antigens. The kinetics of expression of these activation antigens or resti...
Davis, W.C.
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Naessens, Jan
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Brown, W.C.
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Ellis, J.A.
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Hamilton, M.J.
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Canton, G.H.
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Barbosa, J.J.R.
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Ferens, W.
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Bohach, G.A.
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[Analysis of monoclonal antibodies reactive with molecules upregulated or expressed only on activated lymphocytes]
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Analysis of monoclonal antibodies reactive with molecules upregulated or expressed only on activated lymphocytes
After analysis of flowcytometry data on bovine and ovine cells and immunoprecipitation studies, a cluster of seven MAbs-CAPP2 (3W-232), IL-A164 (M430), IL-A166 (3W-226), IVA30 (3W-069), IVA31 (3W-468), IVA38 (3W-349) and IVA125 (3W 528) - was shown to detect bovine and ovine CD41/Cd61 (also known...
Mateo, A.
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Pintado, C.O.
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Lastra, J.de la
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Dusinsky, R.
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Simon, M.
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Naessens, Jan
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Llanes, D.
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[Ruminant cluster CD41/CD61]
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Ruminant cluster CD41/CD61
Naessens, Jan
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Olubayo, R.O.
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Davis, W.C.
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Hopkins, J.
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[Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants]
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Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants