African animal trypanosomiasis (AAT) caused by tsetse fly-transmitted protozoa of the genus Trypanosoma is a major constraint on livestock and agricultural production in Africa and is among the top ten global cattle diseases impacting on the poor. Here we show that a functional genomics approach ...
O'Gorman, G.M.
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Park, S. de
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Hill, E.W.
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Meade, K.G.
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Coussens, P.M.
,
Agaba, Morris
,
Naessens, Jan
,
Kemp, Stephen J.
,
MacHugh, D.E.
,
[Transcriptional profiling of cattle infected with Trypanosoma congolense highlights gene expression signatures underlying trypanotolerance and trypanosusceptibility]
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Transcriptional profiling of cattle infected with Trypanosoma congolense highlights gene expression signatures underlying trypanotolerance and trypanosusceptibility
West African N'Dama cattle have developed a genetic capacity to survive, reproduce and remain productive under trypanosomosis risk. The cellular and molecular bases of this so-called trypanotolerance are not known, but the trait is manifested by the N'Dama's greater capacity to control parasitae...
Naessens, Jan
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Leak, S.G.A.
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Kennedy, D.J.
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Kemp, Stephen J.
,
Teale, A.J.
,
[Responses of bovine chimaeras combining trypanosomosis resistant and susceptible genotypes to experimental infection with Trypanosoma congolense]
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Responses of bovine chimaeras combining trypanosomosis resistant and susceptible genotypes to experimental infection with Trypanosoma congolense
The first visible response in livestock to the bite of a trypanosome-infected tsetse fly is the formation of a localized skin reaction, also known as a chancre. This is an inflammatory response in the skin associated with swelling and an influx of cells. It is thought to be associated with an a...
Naessens, Jan
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Mwangi, D.M.
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Buza, J.
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Moloo, S.K.
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[Local skin reaction (chancre) induced following inoculation of metacyclic trypanosomes in cattle by tsetse flies is dependent on CD4 T lymphocytes]
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Local skin reaction (chancre) induced following inoculation of metacyclic trypanosomes in cattle by tsetse flies is dependent on CD4 T lymphocytes
Development of anaemia in inflammatory diseases is cytokine-mediated. Specifically, the levels of tumour necrosis factor-alpha (TNF-alpha), produced by activated macrophages, are correlated with severity of disease and anaemia in infections and chronic disease. In African trypanosomiasis, anaemia...
Naessens, Jan
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Kitani, H.
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Nakamura, Y.
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Yagi, Y.
,
Sekikawa, K.
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Iraqi, F.A.
,
[TNF-alpha mediates the development of anaemia in a murine Trypanosoma brucei rhodesiense infection, but not the anaemia associated with a murine Trypanosoma congolense infection]
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TNF-alpha mediates the development of anaemia in a murine Trypanosoma brucei rhodesiense infection, but not the anaemia associated with a murine Trypanosoma congolense infection
C57BL/6 mice deficient in one or two copies of the gene for tumor necrosis factor alpha (TNF-alpha) were more susceptible to Trypanosoma congolense infection than their resistant, wild-type counterparts. The number of TNF-alpha genes was correlated with the capacity to control parasitaemia and ...
Naessens, Jan
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Kitani, H.
,
Momotani, E.
,
Sekikawa, K.
,
Nthale, J.M.
,
Iraqi, F.A.
,
[Susceptibility of TNF-alpha-deficient mice to Trypanosoma congolense is not due to a defective antibody response]
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Susceptibility of TNF-alpha-deficient mice to Trypanosoma congolense is not due to a defective antibody response
Naessens, Jan
,
Williams, D.J.L.
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[Characterization and measurement of CD5+ B cells in normal and Trypanosoma congolense- infected cattle]
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Characterization and measurement of CD5+ B cells in normal and Trypanosoma congolense- infected cattle
African buffalo (Syncerus caffer) and trypanotolerant N'Dama cattle were compared for their resistance to overcome Trypanosoma congolense and T. vivax infections. Several compartments from the immune system were followed during the infections and compared between the two species. Several parmaete...
Olubayo, R.O.
,
Moloo, S.K.
,
Naessens, Jan
,
[Comparative parasite development in African buffalo and N'Dama cattle infected with either Trypanosoma congolense or T. vivax]
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Comparative parasite development in African buffalo and N'Dama cattle infected with either Trypanosoma congolense or T. vivax
Mice whose tumor necrosis factor alpha (TNF-) genes were disrupted developed higher levels of parasitemia than wild-type mice following infection with Trypanosoma congolense IL1180 or T. brucei brucei GUTat3.1, confirming the results of earlier studies. To determine whether TNF- directly affects ...
Kitani, H.
,
Black, S.J.
,
Nakamura, Y.
,
Naessens, Jan
,
Murphy, N.
,
Yokomizo, Y.
,
Gibson, John P.
,
Iraqi, F.A.
,
[Recombinant tumor necrosis factor alpha does not inhibit the growth of African trypanosomes in Axenic cultures]
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Recombinant tumor necrosis factor alpha does not inhibit the growth of African trypanosomes in Axenic cultures
To examine differences in cytokine profiles that may confer tolerance/susceptibility to bovine African trypanosomiasis, N'Dama (trypanotolerant, n = 8) and Boran (trypanosusceptible, n = 8) cattle were experimentally challenged with Trypanosoma congolense. Blood samples were collected over a 34-d...
O'Gorman, G.M.
,
Park, S.D.E.
,
Hill, E.W.
,
Meade, K.G.
,
Mitchell, L.C.
,
Agaba, Morris
,
Gibson, John P.
,
Hanotte, Olivier H.
,
Naessens, Jan
,
Kemp, Stephen J.
,
MacHugh, D.E.
,
[Cytokine mRNA profiling of peripheral blood mononuclear cells from trypanotolerant and trypanosusceptible cattle infected with Trypanosoma congolense]
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Cytokine mRNA profiling of peripheral blood mononuclear cells from trypanotolerant and trypanosusceptible cattle infected with Trypanosoma congolense
Trypanosoma congolense is a protozoan parasite that causes severe diseases in livestock. Three major quantative trait loci (QTL), Tir1, Tir2, and Tir3, control the survival time of mice after infection with T. congolense. Congenic mice carrying the C57BL/6 resistance alleles on the A/J background...
Rathkolb, B.
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Noyes, H.A.
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Brass, A.
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Dark, P.
,
Fuchs, H.
,
Gailus-Durner, V.
,
Gibson, John P.
,
Angelis, M.H. de
,
Ogugo, M.
,
Iraqi, F.A.
,
Kemp, Stephen J.
,
Naessens, Jan
,
Pope, M.E.
,
Wolf, E.
,
Agaba, Morris
,
[Clinical chemistry of congenic mice with quantitative trait loci for predicted responses to Trypanosoma congolense infection]
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Clinical chemistry of congenic mice with quantitative trait loci for predicted responses to Trypanosoma congolense infection