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A modified NC membrane-based dot-ELISA was used to detect and differentiate between Trypanosoma brucei, T. congolense and T. simiae procyclics in the midguts of experimentally infected tsetse flies. The modification of the assay consisted of (a) the lysis of T. congolense or T. simiae in NC membr...


Bosompem, K.M.Moloo, S.K.Assoku, R.K.G.Nantulya, V.M.[Detection and differentiation between trypanosome species in experimentally infected tsetse flies (Glossina spp.) using dot-ELISA]Detection and differentiation between trypanosome species in experimentally infected tsetse flies (Glossina spp.) using dot-ELISA

Relationships were evaluated between trypanosome infection as measured by antigen detection enzyme immunoassays (antigen ELISA), anaemia as determined by average packed red cell volume (PCV), and animal performance as assessed by daily weight gain in 99 N'Dama cattle in Gabon exposed to natural t...


Trail, J.C.M.D'Ieteren, G.D.M.Viviani, P.Yangari, G.Nantulya, V.M.[Relationships between trypanosome infection measured by antigen detection enzyme immunoassays, anaemia and growth in trypanotolerant N'Dama cattle]Relationships between trypanosome infection measured by antigen detection enzyme immunoassays, anaemia and growth in trypanotolerant N'Dama cattle
Waitumbi, J.N.Nantulya, V.M.[A comparison of the antigen detection ELISA and parasite detection for diagnosis of Trypanosoma evansi infections in camels]A comparison of the antigen detection ELISA and parasite detection for diagnosis of Trypanosoma evansi infections in camels
Komba, E.Odiit, M.Mbulamberi, D.B.Chimfwembe, E.C.Nantulya, V.M.[Multicentre evaluation of an antigen-detection ELISA for the diagnosis of Trypanosoma brucei rhodesiense sleeping sickness]Multicentre evaluation of an antigen-detection ELISA for the diagnosis of Trypanosoma brucei rhodesiense sleeping sickness

A rapid, visually read, dot-ELISA developed for the detection and differentiation of trypanosome species in tsetse flies (Glossina spp.), was field tested alongside the standard fly dissection method on a ranch in south eastern Kenya. Of 104 G. pallidipes dissected, 2 were found to be infected wi...


Bosompem, K.M.Masake, R.A.Assoku, R.K.G.Opiyo, E.A.Nantulya, V.M.[Field evaluation of a dot-ELISA for the detection and differentiation of trypanosome species in infected tsetse flies (Glossina spp.)]Field evaluation of a dot-ELISA for the detection and differentiation of trypanosome species in infected tsetse flies (Glossina spp.)

The nucleic acid probes that are currently in use detect and distinguish Trypanosoma vivax parasites according to their geographic origin. To eliminate the need for using multiple DNA probes, a study was conducted to evaluate the suitability of a tandemly reiterated sequence which encodes a T. vi...


Masake, R.A.Majiwa, P.A.O.Moloo, S.K.Makau, J.M.Njuguna, J.Maina, M.Kabata, J.M.Ole-MoiYoi, O.K.Nantulya, V.M.[Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction]Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction

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