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For the purpose of constructing yam cDNA libraries, attempts to isolate high quality RNA using several previously reported protocols were unsuccessful. Therefore a protocol was standardized for yam total RNA isolation by using guanidium buffer at the Department of Biology, Virginia Sate Universit...


Narina, S.S.Mohammed, I.Asiedu, RobertMignouna, H.D.[A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction]A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction

Background: Anthracnose (Colletotrichum gloeosporioides) is a major limiting factor in the production of yam (Dioscorea spp.) worldwide. Availability of high quality sequence information is necessary for designing molecular markers associated with resistance. However, very limited sequence inform...


Narina, S.S.Buyyarapu, R.Kottapalli, K.R.Sartie, A.M.Ali, M.I.Asiedu, RobertHodeba, M.Sayre, B.L.Scheffler, B.E.[Generation and analysis of expressed sequence tags (ESTs) for marker development in yam (Dioscorea alata L.)]Generation and analysis of expressed sequence tags (ESTs) for marker development in yam (Dioscorea alata L.)

Greater yam (Dioscorea alata L.), belonging to the family Dioscoreaceae, is a staple food crop of the tropics. It is cultivated for its edible, nutritious, underground tubers. Its productivity declined due to anthracnose disease. There was no sequence information for yam in the public database. T...


Narina, S.S.Montegue, P.Asiedu, RobertMohamed, A.I.[Development of microsatellite markers for anthracnose resistance in greater yam (Dioscorea alata L.) from expressed sequence tags of heterologous crop species]Development of microsatellite markers for anthracnose resistance in greater yam (Dioscorea alata L.) from expressed sequence tags of heterologous crop species

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