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Random clones from a cDNA library made from mRNA purified from dissected salivary glands of feeding female Amblyomma variegatum ticks were subjected to single pass sequence analysis. A total of 3,992 sequences with an average read length of 580 nucleotides have been used to construct a gene index...


Nene, VishvanathLee, D.Quackenbush, J.Skilton, Robert A.Mwaura, S.Gardner, M.J.Bishop, Richard P.[AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum]AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum
Heekin, A.M.Guerrero, F.D.Bendele, K.G.Saldivar, L.Scoles, Glen A.Gondro, C.Nene, VishvanathDjikeng, AppolinaireBrayton, K.A.[Analysis of Babesia bovis infection-induced gene expression changes in larvae from the cattle tick, Rhipicephalus (Boophilus) microplus]Analysis of Babesia bovis infection-induced gene expression changes in larvae from the cattle tick, Rhipicephalus (Boophilus) microplus

Reassociation kinetics and flow cytometry data indicate that ixodid tick genomes are large, relative to most arthropods, containing ≥ 109 base pairs. The molecular basis for this is unknown. We have identified a novel small interspersed element with features of a tRNA-derived SINE, designated Ruk...


Sunter, J.D.Patel, S.P.Skilton, Robert A.Knowles, Donald P.Scoles, Glen A.Nene, VishvanathVilliers, Etienne P. deBishop, Richard P.[A novel SINE family occurs frequently in both genomic DNA and transcribed sequences in ixodid ticks of the arthropod sub-phylum Chelicerata]A novel SINE family occurs frequently in both genomic DNA and transcribed sequences in ixodid ticks of the arthropod sub-phylum Chelicerata

Cattle babesiosis is a tick-borne disease of cattle with the most severe form of the disease caused by the apicomplexan, Babesia bovis. Babesiosis is transmitted to cattle through the bite of infected cattle ticks of the genus Rhipicephalus. The most prevalent species is Rhipicephalus (Boophilus)...


Heekin, A.M.Guerrero, F.D.Bendele, K.G.Saldivar, L.Scoles, Glen A.Dowd, S.E.Gondro, C.Nene, VishvanathDjikeng, AppolinaireBrayton, K.A.[The ovarian transcriptome of the cattle tick, Rhipicephalus (Boophilus) microplus, feeding upon a bovine host infected with Babesia bovis]The ovarian transcriptome of the cattle tick, Rhipicephalus (Boophilus) microplus, feeding upon a bovine host infected with Babesia bovis

Previous studies using monoclonal antibody (mAb) 4C9 specific for a 32 kDa antigen (p32) of Theileria parva demonstrated expression of the antigen on the surface of the sporozoite, making it a potential antigen for sporozoite neutralization. A full-length cDNA encoding the major merozoite/piropla...


Skilton, Robert A.Musoke, A.J.Wells, C.W.Yagi, Y.Nene, VishvanathSpooner, P.R.Gachanja, J.Osaso, J.Bishop, Richard P.Morzaria, S.P.[A 32 kDa surface antigen of Theileria parva: Characterization and immunization studies]A 32 kDa surface antigen of Theileria parva: Characterization and immunization studies

Immunity against the bovine protozoan parasite Theileria parva has previously been shown to be mediated through lysis of parasite-infected cells by MHC class I restricted CD8+ cytotoxic T lymphocytes. It is hypothesized that identification of CTL target schizont antigens will aid the development ...


Graham, S.P.Honda, Y.Pelle, RogerMwangi, D.M.Glew, E.J.Villiers, Etienne P. deShah, TushaarBishop, Richard P.Bruggen, P. van derNene, VishvanathTaracha, E.L.N.[A novel strategy for the identification of antigens that are recognised by bovine MHC class I restricted cytotoxic T cells in a protozoan infection using reverse vaccinology]A novel strategy for the identification of antigens that are recognised by bovine MHC class I restricted cytotoxic T cells in a protozoan infection using reverse vaccinology

Initial comparison of 2 polymorphic, immunodominant molecule (PIM) cDNA sequences from Theileria parva suggested that the PIM genes consist of conserved 5' and 3' termini flanking a central variable region. Further evidence is presented, based on sequence analysis, supporting this general structu...


Toye, Philip G.Gobright, E.I.Nyanjui, J.Nene, VishvanathBishop, Richard P.[Structure and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PlM), a candidate diagnostic antigen of Theileria parva]Structure and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PlM), a candidate diagnostic antigen of Theileria parva

Awino, EliasAye, R.Bertrand, S.Gesharisha, J.Gicheru, N.Jores, JoergLiljander, Anne M.Muriuki, C.Naessens, JanNene, VishvanathNjoroge, T.Nyongesa, S.Pelle, RogerSsajjakambwe, P.Saya, R.Schieck, Elise G.Steinaa, LucillaSvitek, NicholasWasilwa, J.[ILRI vaccine biosciences—Planting the orchard: (ILRI’s Vaccine Platform‐ILVAC)]ILRI vaccine biosciences—Planting the orchard: (ILRI’s Vaccine Platform‐ILVAC)

Tick-borne diseases (TBDs) are a major economic constraint to livestock production in sub-Saharan Africa. ILRI is focussing on developing a range of products, such as vaccines, diagnostics and decision support services to underpin improved control programmes against these diseases. We have develo...


Morzaria, S.P.Katende, J.Musoke, A.J.Nene, VishvanathSkilton, Robert A.Bishop, Richard P.[Development of sero-diagnostic and molecular tools for the control of important tick-borne pathogens of cattle in Africa]Development of sero-diagnostic and molecular tools for the control of important tick-borne pathogens of cattle in Africa

We describe the generation of an auto-annotated index of genes that are expressed in the salivary glands of four-day fed female adult Rhipicephalus appendiculatus ticks. A total of 9162 EST sequences were derived from an uninfected tick cDNA library and 9844 ESTs were from a cDNA library from t...


Nene, VishvanathLee, D.Kang'a, S.Skilton, Robert A.Shah, TushaarVilliers, Etienne P. deMwaura, S.Taylor, D.Quackenbush, J.Bishop, Richard P.[Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva]Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva

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