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Flow cytometry, enzyme-linked immunospot (ELISpot) and cellular cytotoxicity assays are powerful tools for studying the cellular immune response towards intracellular pathogens and vaccines in livestock species. Lymphocytes from immunized animals can be purified using Ficoll-Paque density gradien...


Svitek, NicholasTaracha, E.L.N.Saya, R.Awino, EliasNene, VishvanathSteinaa, Lucilla[Analysis of the cellular immune responses to vaccines]Analysis of the cellular immune responses to vaccines

Grace, DeliaToye, Philip G.Tarawali, Shirley A.Nene, Vishvanath[Vaccines and diagnostics—The case for regional One Health centres of excellence]Vaccines and diagnostics—The case for regional One Health centres of excellence

Globally, malaria remains one of the most important vector-borne diseases despite the extensive use of vector control, including indoor residual spraying (IRS) and insecticide-treated nets (ITNs). These control methods target endophagic vectors, whereas some malaria vectors, such as Anopheles ara...


Poché, R.M.Githaka, Naftaly W.Gool, F. vanKading, R.C.Hartman, D.A.Polyakova, L.Abworo, E.O.Nene, VishvanathLozano-Fuentes, Saul[Preliminary efficacy investigations of oral fipronil against Anopheles arabiensis when administered to Zebu cattle (Bos indicus) under field conditions]Preliminary efficacy investigations of oral fipronil against Anopheles arabiensis when administered to Zebu cattle (Bos indicus) under field conditions

Wildebeest-associated malignant catarrhal fever (WA-MCF), a fatal disease of cattle caused by alcelaphine herpesvirus 1 (AlHV-1), is one of the most important seasonal diseases of cattle in wildebeest endemic areas, with annual incidence reaching 10%. Here we report efficacy of over 80% for a vac...


Cook, Elizabeth A.J.Russell, GeorgeGrant, DawnMutisya, Christine N.Omoto, LazarusDobson, ElizabethLankester, FelixNene, Vishvanath[A randomised vaccine field trial in Kenya demonstrates protection against wildebeest-associated malignant catarrhal fever in cattle]A randomised vaccine field trial in Kenya demonstrates protection against wildebeest-associated malignant catarrhal fever in cattle

The intracellular protozoan parasite Theileria parva transforms bovine lymphocytes inducing uncontrolled proliferation. Proteins released from the parasite are assumed to contribute to phenotypic changes of the host cell and parasite persistence. With 85 members, genes encoding subtelomeric varia...


Schmuckli-Maurer, J.Casanova, C.S.Affentranger, S.Kang'a, S.Nene, VishvanathKatzer, F.McKeever, Declan J.Müller, JoachimBishop, Richard P.Arnab Pain, A.Dobbelaere, D.A.E.[Expression analysis of the Theileria parva Subtelomere-encoded Variable Secreted Protein gene family]Expression analysis of the Theileria parva Subtelomere-encoded Variable Secreted Protein gene family

We present complete sequences of the mitochondrial genomes for two important mosquitoes, Aedes aegypti and Culex quinquefasciatus, that are major vectors of dengue virus and lymphatic filariasis, respectively. The A. aegypti mitochondrial genome is 16,655 bp in length and that of Culex quinquefas...


Behura, S.K.Lobo, N.F.Haas, B.Bruyn, B. deLovin, D.D.Shumway, M.F.Puiu, D.Romero Severson, J.Nene, VishvanathSeverson, D.W.[Complete sequences of mitochondria genomes of Aedes aegypti and Culex quinquefasciatus and comparative analysis of mitochondrial DNA fragments inserted in the nuclear genomes]Complete sequences of mitochondria genomes of Aedes aegypti and Culex quinquefasciatus and comparative analysis of mitochondrial DNA fragments inserted in the nuclear genomes

In this study, we investigated the immunological effects of bovine heat shock protein 70 (HSP70) on the major Theileria sergenti surface protein (p33). The gene encoding p33 was expressed as a fusion protein with bovine HSP70 from a plasmid vector. The adjuvant function of HSP70 on p33 was evalua...


Jeong, W.Kweon, C.H.Kwang, S.W.Hu Suk LeeXu, Y.Lu, C.Zhang, S.Nene, Vishvanath[Adjuvant effect of bovine heat shock protein 70 on piroplasm surface protein, p33, of Theileria sergenti]Adjuvant effect of bovine heat shock protein 70 on piroplasm surface protein, p33, of Theileria sergenti

Resistance or susceptibility of the snail host Biomphalaria glabrata to Schistosoma mansoni is determined by the genetics of both the snail and parasite. Although Mendelian genetics governs adult resistance to infection, juvenile resistance and susceptibility are complex traits. In this study, su...


Ittiprasert, W.Miller, A.Myers, J.Nene, VishvanathEl-Sayed, N.M.Knight, M.[Identification of immediate response genes dominantly expressed in juvenile resistant and susceptible Biomphalaria glabrata snails upon exposure to Schistosoma mansoni]Identification of immediate response genes dominantly expressed in juvenile resistant and susceptible Biomphalaria glabrata snails upon exposure to Schistosoma mansoni
Bishop, Richard P.Musoke, A.J.Skilton, Robert A.Morzaria, S.P.Gardner, M.Nene, Vishvanath[Theileria: Life cycle stages associated with the ixodid tick vector]Theileria: Life cycle stages associated with the ixodid tick vector
Bishop, Richard P.Musoke, A.J.Skilton, Robert A.Odongo, D.Scoles, Glen A.Knowles, D.Nene, Vishvanath[Approaches to improved understanding of vector parasite interaction within the genus Theileria and their Ixodid tick vectors]Approaches to improved understanding of vector parasite interaction within the genus Theileria and their Ixodid tick vectors

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