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Enhancement of the induction of cytotoxic T-cell responses by immunostimulatory CpG oligodeoxynucleotides has been described in humans and mouse models. The present study attempted to address whether CpG has a similar effect in cattle. Immunisation of cattle with a recombinant form of the polymor...


Graham, S.P.Saya, R.Awino, EliasNgugi, D.Nyanjui, J.K.Hecker, R.Taracha, E.L.N.Nene, Vishvanath[Immunostimulatory CpG oligodeoxynucleotides enhance the induction of bovine CD4+ cytotoxic T-lymphocyte responses against the polymorphic immunodominant molecule of the protozoan parasite Theileria parva]Immunostimulatory CpG oligodeoxynucleotides enhance the induction of bovine CD4+ cytotoxic T-lymphocyte responses against the polymorphic immunodominant molecule of the protozoan parasite Theileria parva

Full-length recombinant versions of p67, the 709 amino acid major surface protein of Theileria parva sporozoites, induce immunity to East Coast fever (ECF) in cattle. We show that a soluble Escherichia coli recombinant version of p67 (p67635), in which a prokaryotic signal peptide replaces the e...


Bishop, Richard P.Nene, VishvanathStaeyert, J.Rowlands, G.J.Nyanjui, J.Osaso, J.Morzaria, S.P.Musoke, A.J.[Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites]Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites

Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67DeltaSS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and th...


Kaba, S.A.Musoke, A.J.Schaap, D.Schetters, T.Rowlands, G.J.Vermeulen, A.N.Nene, VishvanathVlak, J.M.Oers, M.M. van[Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle]Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle
Gardner, M.J.Bishop, Richard P.Shah, TushaarVilliers, Etienne P. deCarlton, J.M.Hall, N.Ren, Q.Paulsen, I.T.Pain, A.Berriman, M.Wilson, R.J.M.Sato, S.Ralph, S.A.Mann, D.J.Xiong, Z.Shallom, S.J.Weidman, J.Jiang, L.Lynn,, J.Weaver, B.Shoaibi, A.Domingo, A.R.Wasawo, D.Crabtree, J.Wortman, J.R.Haas, B.Angiuoli, S.V.Creasy, T.H.Lu, C.Suh, B.Silva, J.C.Utterback, T.R.Feldblyum, T.V.Pertea, M.Allen, J.Nierman, W.C.Taracha, E.L.N.Salzberg, S.L.White, O.R.Fitzhugh, H.A.Morzaria, S.P.Venter, J. CraigFraser, C.M.Nene, Vishvanath[Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytes]Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytes

East Coast fever (ECF) in cattle is caused by the tick-borne protozoan parasite Theileria parva. The major sporozoite surface antigen of T. parva (p67) is an important candidate for inclusion in a subunit vaccine. Recently, we reported the expression and production of different parts of p67 as f...


Kaba, S.A.Schaap, D.Roode, E.C.Nene, VishvanathMusoke, A.J.Vlak, J.M.Oers, M.M. van[Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens]Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens

Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one i...


Musoke, A.J.Rowlands, G.J.Nene, VishvanathNyanjui, J.Katende, J.Spooner, P.R.Mwaura, S.Odongo, D.Nkonge, C.Mbogo, S.Bishop, Richard P.Morzaria, S.P.[Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge]Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge
Pain, A.Renauld, H.Berriman, M.Murphy, L.Yeats, C.A.Weir, W.Kerhornou, A.Aslett, M.Bishop, Richard P.Bouchier, C.Bouchier, C.Cochet, M.Coulson, R.M.R.Cronin, A.Villiers, Etienne P. deFraser, A.Fosker, N.Gardner, M.Goble, A.Griffiths-Jones, S.Harris, D.E.Katzer, F.Larke, N.Lord, A.Maser, P.McKellar, S.Mooney, P.Morton, F.Nene, VishvanathO'Neil, S.Price, C.Quail, M.A.Rabbinowitsch, E.Rawlings, N.D.Rutter, S.Saunders, D.Seeger, K.Shah, TushaarSquares, R.Squares, S.Tivey, A.Walker, A.R.Woodward, J.Dobbelaere, D.A.E.Langsley, G.Rajandream, M.A.McKeever, Declan J.Shiels, B.Tait, A.Barrell, B.Hall, N.[Genome of the host-cell transforming parasite Theileria annulata compared with T. parva]Genome of the host-cell transforming parasite Theileria annulata compared with T. parva
Nene, VishvanathStaal, Steven J.Tarawali, Shirley A.Johnson, Nancy L.Notenbaert, An Maria Omer[Livestock goods and bads: Perspectives from ILRI research]Livestock goods and bads: Perspectives from ILRI research

Improvement of the livestock industry in many developing countries has been severely constrained by tick-borne diseases. The major protozoan pathogens responsible for these diseases in cattle are Theileria parva and B. annulata and Babesia bigemina and B. bovis. East Coast fever is of great econo...


Musoke, A.J.Morzaria, S.P.Nene, Vishvanath[Subunit vaccines for the control of Theileria parva]Subunit vaccines for the control of Theileria parva

Kemp, Stephen J.Nene, Vishvanath[Biosciences research at the International Livestock Research Institute (ILRI)]Biosciences research at the International Livestock Research Institute (ILRI)

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