Mycoplasma mycoides subsp. capri (Mmc) and subsp. mycoides (Mmm) are important ruminant pathogens worldwide causing diseases such as pleuropneumonia, mastitis and septicaemia. They express galactofuranose residues on their surface, but their role in pathogenesis has not yet been determined. The M...
Schieck, Elise G.
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Lartigue, C.
,
Frey, J.
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Vozza, N.
,
Hegermann, J.
,
Miller, R.A.
,
Valguarnera, E.
,
Muriuki, C.
,
Meens, J.
,
Nene, Vishvanath
,
Naessens, Jan
,
Weber, J.
,
Lowary, T.L.
,
Vashee, S.
,
Feldman, M.F.
,
Jores, Joerg
,
[Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance]
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Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance
We have recently shown that the BoLA-A18 variant haplotype (BoLA-6*01302) is more prevalent than the BoLA-A18 haplotype (BoLA-6*01301) in a sample of Holstein/Friesian cattle in Kenya. These MHC class I allelic variants differ by a single amino acid polymorphism (Glu97 to Leu97) in the peptide-bi...
Svitek, Nicholas
,
Awino, Elias
,
Nene, Vishvanath
,
Steinaa, Lucilla
,
[BoLA-6*01301 and BoLA-6*01302, two allelic variants of the A18 haplotype, present the same epitope from the Tp1 antigen of Theileria parva]
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BoLA-6*01301 and BoLA-6*01302, two allelic variants of the A18 haplotype, present the same epitope from the Tp1 antigen of Theileria parva
Transcriptome analysis can provide useful data for refining genome sequence annotation. Application of massively parallel signature sequencing (MPSS) revealed reproducible transcription, in multiple MPSS cycles, from 73% of computationally predicted genes in the Theileria parva schizont lifecycle...
Shah, Tushaar
,
Villiers, Etienne P. de
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Nene, Vishvanath
,
Hass, B.
,
Taracha, E.A.
,
Gardner, J.M.
,
Sansom, C.
,
Pelle, Roger
,
Bishop, Richard P.
,
[Using the transcriptome to annotate the genome revisited: application of massively parallel signature sequencing (MPSS)]
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Using the transcriptome to annotate the genome revisited: application of massively parallel signature sequencing (MPSS)
We describe the generation of an auto-annotated index of genes that are expressed in the salivary glands of four-day fed female adult Rhipicephalus appendiculatus ticks. A total of 9162 EST sequences were derived from an uninfected tick cDNA library and 9844 ESTs were from a cDNA library from tic...
Nene, Vishvanath
,
Lee, D.
,
Kang'a, S.
,
Skilton, Robert A.
,
Shah, Tushaar
,
Villiers, Etienne P. de
,
Mwaura, S.
,
Taylor, D.
,
Quackenbush, J.
,
Bishop, Richard P.
,
[Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva]
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Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva
Reassociation kinetics and flow cytometry data indicate that ixodid tick genomes are large, relative to most arthropods, containing ≥ 109 base pairs. The molecular basis for this is unknown. We have identified a novel small interspersed element with features of a tRNA-derived SINE, designated Ruk...
Sunter, J.D.
,
Patel, S.P.
,
Skilton, Robert A.
,
Knowles, Donald P.
,
Scoles, Glen A.
,
Nene, Vishvanath
,
Villiers, Etienne P. de
,
Bishop, Richard P.
,
[A novel SINE family occurs frequently in both genomic DNA and transcribed sequences in ixodid ticks of the arthropod sub-phylum Chelicerata]
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A novel SINE family occurs frequently in both genomic DNA and transcribed sequences in ixodid ticks of the arthropod sub-phylum Chelicerata
Previous studies using monoclonal antibody (mAb) 4C9 specific for a 32 kDa antigen (p32) of Theileria parva demonstrated expression of the antigen on the surface of the sporozoite, making it a potential antigen for sporozoite neutralization. A full-length cDNA encoding the major merozoite/piropla...
Skilton, Robert A.
,
Musoke, A.J.
,
Wells, C.W.
,
Yagi, Y.
,
Nene, Vishvanath
,
Spooner, P.R.
,
Gachanja, J.
,
Osaso, J.
,
Bishop, Richard P.
,
Morzaria, S.P.
,
[A 32 kDa surface antigen of Theileria parva: Characterization and immunization studies]
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A 32 kDa surface antigen of Theileria parva: Characterization and immunization studies
Immunity against the bovine protozoan parasite Theileria parva has previously been shown to be mediated through lysis of parasite-infected cells by MHC class I restricted CD8+ cytotoxic T lymphocytes. It is hypothesized that identification of CTL target schizont antigens will aid the development ...
Graham, S.P.
,
Honda, Y.
,
Pelle, Roger
,
Mwangi, D.M.
,
Glew, E.J.
,
Villiers, Etienne P. de
,
Shah, Tushaar
,
Bishop, Richard P.
,
Bruggen, P. van der
,
Nene, Vishvanath
,
Taracha, E.L.N.
,
[A novel strategy for the identification of antigens that are recognised by bovine MHC class I restricted cytotoxic T cells in a protozoan infection using reverse vaccinology]
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A novel strategy for the identification of antigens that are recognised by bovine MHC class I restricted cytotoxic T cells in a protozoan infection using reverse vaccinology
Initial comparison of 2 polymorphic, immunodominant molecule (PIM) cDNA sequences from Theileria parva suggested that the PIM genes consist of conserved 5' and 3' termini flanking a central variable region. Further evidence is presented, based on sequence analysis, supporting this general structu...
Toye, Philip G.
,
Gobright, E.I.
,
Nyanjui, J.
,
Nene, Vishvanath
,
Bishop, Richard P.
,
[Structure and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PlM), a candidate diagnostic antigen of Theileria parva]
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Structure and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PlM), a candidate diagnostic antigen of Theileria parva
Awino, Elias
,
Aye, R.
,
Bertrand, S.
,
Gesharisha, J.
,
Gicheru, N.
,
Jores, Joerg
,
Liljander, Anne M.
,
Muriuki, C.
,
Naessens, Jan
,
Nene, Vishvanath
,
Njoroge, T.
,
Nyongesa, S.
,
Pelle, Roger
,
Ssajjakambwe, P.
,
Saya, R.
,
Schieck, Elise G.
,
Steinaa, Lucilla
,
Svitek, Nicholas
,
Wasilwa, J.
,
[ILRI vaccine biosciences—Planting the orchard: (ILRI’s Vaccine Platform‐ILVAC)]
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ILRI vaccine biosciences—Planting the orchard: (ILRI’s Vaccine Platform‐ILVAC)