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Wildebeest-associated malignant catarrhal fever (WA-MCF), a fatal disease of cattle caused by alcelaphine herpesvirus 1 (AlHV-1), is one of the most important seasonal diseases of cattle in wildebeest endemic areas, with annual incidence reaching 10%. Here we report efficacy of over 80% for a vac...


Cook, Elizabeth A.J.Russell, GeorgeGrant, DawnMutisya, Christine N.Omoto, LazarusDobson, ElizabethLankester, FelixNene, Vishvanath[A randomised vaccine field trial in Kenya demonstrates protection against wildebeest-associated malignant catarrhal fever in cattle]A randomised vaccine field trial in Kenya demonstrates protection against wildebeest-associated malignant catarrhal fever in cattle

Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p15...


Katende, J.Morzaria, S.P.Toye, Philip G.Skilton, Robert A.Nene, VishvanathNkonge, C.Musoke, A.J.[An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule]An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule

The 'Muguga cocktail' which is composed of three Theileria parva stocks Muguga. Kiambu 5 and Serengeti-transformed has been used extensively for live vaccination against East Coast fever in cattle in eastern, central and southern Africa. Herein we describe the molecular characterization of the T....


Bishop, Richard P.Geysen, D.Spooner, P.R.Skilton, Robert A.Nene, VishvanathDolan, T.T.Morzaria, S.P.[Molecular and immunological characterisation of Theileria parva stocks which are components of the Muguga cocktail used for vaccination against East Coast fever in cattle]Molecular and immunological characterisation of Theileria parva stocks which are components of the Muguga cocktail used for vaccination against East Coast fever in cattle

Major histocompatibility complex (MHC) class I restricted cellular immune responses play an important role in immunity to intracellular pathogens. By binding antigenic peptides and presenting them to T cells, class I molecules impose significant selection on the targets of immune responses. Candi...


Russell, G.C.Oliver, R.A.Craigmile, S.Nene, VishvanathGlass, E.J.[Functional expression of a bovine major histocompatibility complex class I gene in transgenic mice]Functional expression of a bovine major histocompatibility complex class I gene in transgenic mice

Enhancement of the induction of cytotoxic T-cell responses by immunostimulatory CpG oligodeoxynucleotides has been described in humans and mouse models. The present study attempted to address whether CpG has a similar effect in cattle. Immunisation of cattle with a recombinant form of the polymor...


Graham, S.P.Saya, R.Awino, EliasNgugi, D.Nyanjui, J.K.Hecker, R.Taracha, E.L.N.Nene, Vishvanath[Immunostimulatory CpG oligodeoxynucleotides enhance the induction of bovine CD4+ cytotoxic T-lymphocyte responses against the polymorphic immunodominant molecule of the protozoan parasite Theileria parva]Immunostimulatory CpG oligodeoxynucleotides enhance the induction of bovine CD4+ cytotoxic T-lymphocyte responses against the polymorphic immunodominant molecule of the protozoan parasite Theileria parva

Full-length recombinant versions of p67, the 709 amino acid major surface protein of Theileria parva sporozoites, induce immunity to East Coast fever (ECF) in cattle. We show that a soluble Escherichia coli recombinant version of p67 (p67635), in which a prokaryotic signal peptide replaces the e...


Bishop, Richard P.Nene, VishvanathStaeyert, J.Rowlands, G.J.Nyanjui, J.Osaso, J.Morzaria, S.P.Musoke, A.J.[Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites]Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites

Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67DeltaSS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and th...


Kaba, S.A.Musoke, A.J.Schaap, D.Schetters, T.Rowlands, G.J.Vermeulen, A.N.Nene, VishvanathVlak, J.M.Oers, M.M. van[Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle]Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle

Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one i...


Musoke, A.J.Rowlands, G.J.Nene, VishvanathNyanjui, J.Katende, J.Spooner, P.R.Mwaura, S.Odongo, D.Nkonge, C.Mbogo, S.Bishop, Richard P.Morzaria, S.P.[Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge]Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge

Previous data showed that six out of a group of nine cattle inoculated with NS1-p67, a recombinant form of a 67-KDa Theileria parva sporozoite surface protein, were immune to East Coast fever. This bacterialy expressed antigen encoded all 709 amino acid residues of p67 fused to the C-terminal end...


Nene, VishvanathInumaru, S.McKeever, Declan J.Morzaria, S.P.Shaw, M.Musoke, A.J.[Characterization of an insect cell-derived Theileria parva sporozoite vaccine antigen and immunogenicity in cattle]Characterization of an insect cell-derived Theileria parva sporozoite vaccine antigen and immunogenicity in cattle

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