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The size and repetitive nature of the Rhipicephalus microplus genome makes obtaining a full genome sequence fiscally and technically problematic. To selectively obtain gene-enriched regions of this tick's genome, Cot filtration was performed, and Cot-filtered DNA was sequenced via 454 FLX pyroseq...


Guerrero, F.D.Moolhuijzen, P.Peterson, D.G.Bidwell, S.Caler, E.Bellgard, M.Nene, VishvanathDjikeng, Appolinaire[Reassociation kinetics-based approach for partial genome sequencing of the cattle tick, Rhipicephalus (Boophilus) microplus]Reassociation kinetics-based approach for partial genome sequencing of the cattle tick, Rhipicephalus (Boophilus) microplus

Massively parallel signature sequencing (MPSS) was used to analyze the transcriptome of the intracellular protozoan Theileria parva. In total 1 095 000, 20 bp sequences representing 4371 different signatures were generated from T.parva schizonts. Reproducible signatures were identified within 73%...


Bishop, Richard P.Shah, TushaarPelle, RogerHoyle, D.Pearson, T.Haines, L.Brass, A.Hulme, H.Graham, S.P.Taracha, E.L.N.Lu, C.Hass, B.Wortman, J.White, O.Gardner, M.J.Nene, VishvanathVilliers, Etienne P. de[Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage]Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage

The intracellular protozoan parasite Theileria parva transforms bovine lymphocytes inducing uncontrolled proliferation. Proteins released from the parasite are assumed to contribute to phenotypic changes of the host cell and parasite persistence. With 85 members, genes encoding subtelomeric varia...


Schmuckli-Maurer, J.Casanova, C.S.Affentranger, S.Kang'a, S.Nene, VishvanathKatzer, F.McKeever, Declan J.Müller, JoachimBishop, Richard P.Arnab Pain, A.Dobbelaere, D.A.E.[Expression analysis of the Theileria parva Subtelomere-encoded Variable Secreted Protein gene family]Expression analysis of the Theileria parva Subtelomere-encoded Variable Secreted Protein gene family

We present complete sequences of the mitochondrial genomes for two important mosquitoes, Aedes aegypti and Culex quinquefasciatus, that are major vectors of dengue virus and lymphatic filariasis, respectively. The A. aegypti mitochondrial genome is 16,655 bp in length and that of Culex quinquefas...


Behura, S.K.Lobo, N.F.Haas, B.Bruyn, B. deLovin, D.D.Shumway, M.F.Puiu, D.Romero Severson, J.Nene, VishvanathSeverson, D.W.[Complete sequences of mitochondria genomes of Aedes aegypti and Culex quinquefasciatus and comparative analysis of mitochondrial DNA fragments inserted in the nuclear genomes]Complete sequences of mitochondria genomes of Aedes aegypti and Culex quinquefasciatus and comparative analysis of mitochondrial DNA fragments inserted in the nuclear genomes

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