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Musoke, A.J.Morzaria, S.P.Nkonge, C.Jones, E.Nene, Vishvanath[A recombinant sporozoite surface antigen of Theileria parva induces protection in cattle]A recombinant sporozoite surface antigen of Theileria parva induces protection in cattle

The major sporozoite surface antigen of Theileria annulata (SPAG-1) is a candidate for inclusion in a subunit vaccine. In this paper we summarize the results of 4 vaccination experiments using recombinant SPAG-1 expressed in different systems and presented in different adjuvants. The antigen has ...


Boulter, N.Brown, D.Wilkie, G.Williamson, S.Kirvar, E.Knight, P.Glass, E.Campbell, J.Morzaria, S.P.Nene, VishvanathMusoke, A.J.Oliveria, C. deGubbels, M.J.Jongejan, F.Hall, R.[Evaluation of recombinant sporozoite antigen SPAG-1 as a vaccine candidate against Theileria annulata by the use of different delivery systems]Evaluation of recombinant sporozoite antigen SPAG-1 as a vaccine candidate against Theileria annulata by the use of different delivery systems

Immunity to the bovine apicomplexan parasite Theileria parva is associated with MHC-I restricted CD8+ T cell responses directed against the intralymphocytic schizont stage of the parasite. A number of schizont-stage antigens that are targets of CD8+ T cell responses from immune animals have been ...


Tinega, A.N.Pelle, RogerKang'a, S.Gicheru, M.M.Taracha, E.L.N.Nene, VishvanathGraham, S.P.[Fusion of a cell penetrating peptide from HIV-1 TAT to the Theileria parva antigen Tp2 enhances the stimulation of bovine CD8+ T cell responses]Fusion of a cell penetrating peptide from HIV-1 TAT to the Theileria parva antigen Tp2 enhances the stimulation of bovine CD8+ T cell responses

East Coast fever is a lymphoproliferative disease caused by the tick-borne protozoan parasite Theileria parva. The sporozoite stage of this parasite, harboured and released from the salivary glands of the tick Rhipicephalus appendiculatus during feeding, invades and establishes infection in bovin...


Nyagwange, JamesTijhaar, EdwinTernette, NicolaMobegi, FredrickTretina, KyleSilva, Joana C.Pelle, RogerNene, Vishvanath[Characterization of the Theileria parva sporozoite proteome]Characterization of the Theileria parva sporozoite proteome

We have developed a polymerase chain reaction-sequence-specific primers-restriction fragment length polymorphism (PCR-SSP-RFLP) method to rapidly differentiate between the A18 and A18 variant (v) BoLA haplotypes and between A14 and A15/A15v BoLA haplotypes in Holstein/Friesian cattle. We used pub...


Svitek, NicholasNzau, Benjamin M.Steinaa, LucillaNene, Vishvanath[A method to discriminate between closely related bovine major histocompatibility complex class I alleles by combining established PCR-SSP assays with RFLPs]A method to discriminate between closely related bovine major histocompatibility complex class I alleles by combining established PCR-SSP assays with RFLPs

Theileria parva is a protozoan parasite that causes the disease East Coast fever in cattle which results in major economic losses in Eastern, Central and Southern Africa. Efforts to generate vaccines involve measurements of cytotoxic activity since CD8 cells are believed to be responsible for pro...


Wendoh, J.Waihenya, R.Saya, R.Awino, EliasNene, VishvanathSteinaa, Lucilla[Perforin expression in Theileria parva specific Cytotoxic T Cells correlates with Cytotoxicity]Perforin expression in Theileria parva specific Cytotoxic T Cells correlates with Cytotoxicity

T cell responses contribute to immunity against many intracellular infections. There is, for example, strong evidence that major histocompatibility complex (MHC) class I-restricted cytotoxic T lymphocytes (CTLs) play an essential role in mediating immunity to East Coast fever (ECF), a fatal lymph...


Nene, VishvanathSvitek, NicholasToye, Philip G.Golde, W.T.Barlow, J.Buus, SorenNielsen, Morten[Designing bovine T cell vaccines via reverse immunology]Designing bovine T cell vaccines via reverse immunology

As it feeds upon cattle, Rhipicephalus (Boophilus) microplus is capable of transmitting a number of pathogenic organisms, including the apicomplexan hemoparasite Babesia bovis, a causative agent of bovine babesiosis. The R. microplus female gut transcriptome was studied for two cohorts: adult fem...


Heekin, A.M.Guerrero, F.D.Bendele, K.G.Saldivar, L.Scoles, Glen A.Dowd, S.E.Gondro, C.Nene, VishvanathDjikeng, AppolinaireBrayton, K.A.[Gut transcriptome of replete adult female cattle ticks, Rhipicephalus (Boophilus) microplus, feeding upon a Babesia bovis-infected bovine host]Gut transcriptome of replete adult female cattle ticks, Rhipicephalus (Boophilus) microplus, feeding upon a Babesia bovis-infected bovine host

Peptide-major histocompatibility complex (p-MHC) class I tetramer complexes have facilitated the early detection and functional characterisation of epitope specific CD8+ cytotoxic T lymphocytes (CTL). Here, we report on the generation of seven recombinant bovine leukocyte antigens (BoLA) and reco...


Svitek, NicholasHansen, A.M.Steinaa, LucillaSaya, R.Awino, EliasNielsen, MortenBuus, SorenNene, Vishvanath[Use of “one-pot, mix-and-read” peptide-MHC class I tetramers and predictive algorithms to improve detection of cytotoxic T lymphocyte responses in cattle]Use of “one-pot, mix-and-read” peptide-MHC class I tetramers and predictive algorithms to improve detection of cytotoxic T lymphocyte responses in cattle
Orono, S.A.Gitao, G.C.Mpatswenumugabo, J.P.Chepkwony, M.Mutisya, Christine N.Okoth, Edward A.Bronsvoort, B.M.C. deRussell, G.C.Nene, VishvanathCook, Elizabeth A.J.[Field validation of clinical and laboratory diagnosis of wildebeest associated malignant catarrhal fever in cattle]Field validation of clinical and laboratory diagnosis of wildebeest associated malignant catarrhal fever in cattle

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