Current serological tests for Babesia bigemina use semipurified merozoite antigens derived from infected erythrocytes. One of the major drawbacks of these tests is that antigen quality can vary from batch to batch. Since the quality of the antigen contributes to the sensitivity and specificity of...
Tebele, N.
,
Skilton, Robert A.
,
Katende, J.
,
Wells, C.W.
,
Nene, Vishvanath
,
McElwain, T.F.
,
Morzaria, S.P.
,
Musoke, A.J.
,
[Cloning, characterization and expression of a 200 Kilodalton diagnostic antigen of Babesia bigemina]
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Cloning, characterization and expression of a 200 Kilodalton diagnostic antigen of Babesia bigemina
Protein components of the cement cone of ixodid ticks are candidates for inclusion in vaccines against tick infestation, since they are essential for tick attachment and feeding. We describe here the cloning of a cDNA encoding a 36 kDa protein, designated Rhipicephalus Immuno-dominant Molecule 36...
Bishop, Richard P.
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Lambson, B.
,
Wells, C.
,
Pandit, P.
,
Osaso, J.
,
Nkonge, C.
,
Morzaria, S.P.
,
Musoke, A.J.
,
Nene, Vishvanath
,
[A cement protein of the tick Rhipicephalus appendiculatus, located in the secretory e cell granules of the type III salivary gland acini, induces strong antibody responses in cattle]
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A cement protein of the tick Rhipicephalus appendiculatus, located in the secretory e cell granules of the type III salivary gland acini, induces strong antibody responses in cattle
Theileria are tick-transmitted, haemoprotozoan parasites infecting wild and domestic ungulates throughout many areas of the world. The most economically important species are T. parva and T. annulata which are pathogenic to cattle, and T. lestoquardi (syn hirci) which is pathogenic to sheep and g...
Skilton, Robert A.
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Musoke, A.J.
,
Nene, Vishvanath
,
Wasawo, D.P.S.
,
Wells, C.W.
,
Spooner, P.R.
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Bishop, Richard P.
,
Osaso, J.
,
Nkonge, C.
,
Latif, A.
,
Morzaria, S.P.
,
[Molecular characterisation of a Theileria lestoquardi gene encoding a candidate sporozoite vaccine antigen]
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Molecular characterisation of a Theileria lestoquardi gene encoding a candidate sporozoite vaccine antigen
Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p15...
Katende, J.
,
Morzaria, S.P.
,
Toye, Philip G.
,
Skilton, Robert A.
,
Nene, Vishvanath
,
Nkonge, C.
,
Musoke, A.J.
,
[An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule]
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An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule
Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67DeltaSS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and th...
Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one i...
Musoke, A.J.
,
Rowlands, G.J.
,
Nene, Vishvanath
,
Nyanjui, J.
,
Katende, J.
,
Spooner, P.R.
,
Mwaura, S.
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Odongo, D.
,
Nkonge, C.
,
Mbogo, S.
,
Bishop, Richard P.
,
Morzaria, S.P.
,
[Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge]
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Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge
A stage-specific surface antigen of Theileria parva, p67, is the basis for the development of an anti-sporozoite vaccine for the control of East Coast fever (ECF) in cattle. By Pepscan analysis with a series of overlapping synthetic p67 peptides, the antigen was shown to contain five distinct lin...
Nene, Vishvanath
,
Gobright, E.I.
,
Bishop, Richard P.
,
Morzaria, S.P.
,
Musoke, A.J.
,
[Linear peptide specificity of bovine antibody responses to p67 of Theileria parva and sequence diversity of sporozoite-neutralising epitopes: Implications for a vaccine]
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Linear peptide specificity of bovine antibody responses to p67 of Theileria parva and sequence diversity of sporozoite-neutralising epitopes: Implications for a vaccine