We have recently shown that the BoLA-A18 variant haplotype (BoLA-6*01302) is more prevalent than the BoLA-A18 haplotype (BoLA-6*01301) in a sample of Holstein/Friesian cattle in Kenya. These MHC class I allelic variants differ by a single amino acid polymorphism (Glu97 to Leu97) in the peptide-bi...
Svitek, Nicholas
,
Awino, Elias
,
Nene, Vishvanath
,
Steinaa, Lucilla
,
[BoLA-6*01301 and BoLA-6*01302, two allelic variants of the A18 haplotype, present the same epitope from the Tp1 antigen of Theileria parva]
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BoLA-6*01301 and BoLA-6*01302, two allelic variants of the A18 haplotype, present the same epitope from the Tp1 antigen of Theileria parva
Reassociation kinetics and flow cytometry data indicate that ixodid tick genomes are large, relative to most arthropods, containing ≥ 109 base pairs. The molecular basis for this is unknown. We have identified a novel small interspersed element with features of a tRNA-derived SINE, designated Ruk...
Sunter, J.D.
,
Patel, S.P.
,
Skilton, Robert A.
,
Knowles, Donald P.
,
Scoles, Glen A.
,
Nene, Vishvanath
,
Villiers, Etienne P. de
,
Bishop, Richard P.
,
[A novel SINE family occurs frequently in both genomic DNA and transcribed sequences in ixodid ticks of the arthropod sub-phylum Chelicerata]
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A novel SINE family occurs frequently in both genomic DNA and transcribed sequences in ixodid ticks of the arthropod sub-phylum Chelicerata
Previous studies using monoclonal antibody (mAb) 4C9 specific for a 32 kDa antigen (p32) of Theileria parva demonstrated expression of the antigen on the surface of the sporozoite, making it a potential antigen for sporozoite neutralization. A full-length cDNA encoding the major merozoite/piropla...
Skilton, Robert A.
,
Musoke, A.J.
,
Wells, C.W.
,
Yagi, Y.
,
Nene, Vishvanath
,
Spooner, P.R.
,
Gachanja, J.
,
Osaso, J.
,
Bishop, Richard P.
,
Morzaria, S.P.
,
[A 32 kDa surface antigen of Theileria parva: Characterization and immunization studies]
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A 32 kDa surface antigen of Theileria parva: Characterization and immunization studies
Immunity against the bovine protozoan parasite Theileria parva has previously been shown to be mediated through lysis of parasite-infected cells by MHC class I restricted CD8+ cytotoxic T lymphocytes. It is hypothesized that identification of CTL target schizont antigens will aid the development ...
Graham, S.P.
,
Honda, Y.
,
Pelle, Roger
,
Mwangi, D.M.
,
Glew, E.J.
,
Villiers, Etienne P. de
,
Shah, Tushaar
,
Bishop, Richard P.
,
Bruggen, P. van der
,
Nene, Vishvanath
,
Taracha, E.L.N.
,
[A novel strategy for the identification of antigens that are recognised by bovine MHC class I restricted cytotoxic T cells in a protozoan infection using reverse vaccinology]
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A novel strategy for the identification of antigens that are recognised by bovine MHC class I restricted cytotoxic T cells in a protozoan infection using reverse vaccinology
In this study, we investigated the immunological effects of bovine heat shock protein 70 (HSP70) on the major Theileria sergenti surface protein (p33). The gene encoding p33 was expressed as a fusion protein with bovine HSP70 from a plasmid vector. The adjuvant function of HSP70 on p33 was evalua...
Jeong, W.
,
Kweon, C.H.
,
Kwang, S.W.
,
Hu Suk Lee
,
Xu, Y.
,
Lu, C.
,
Zhang, S.
,
Nene, Vishvanath
,
[Adjuvant effect of bovine heat shock protein 70 on piroplasm surface protein, p33, of Theileria sergenti]
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Adjuvant effect of bovine heat shock protein 70 on piroplasm surface protein, p33, of Theileria sergenti
Theileria parva, a tick-transmitted protozoan parasite related to Plasmodium spp., causes the disease East Coast fever, an acute and usually fatal lymphoproliferative disorder of cattle in Africa. Previous studies using sera from cattle that have survived infection identified a polymorphic immuno...
Toye, Philip G.
,
Metzelaar, M.J.
,
Wijngaard, P.L.J.
,
Nene, Vishvanath
,
Iams, K.P.
,
Roose, J.
,
Nyanjui, J.K.
,
Gobright, E.I.
,
Musoke, A.J.
,
Clevers, H.C.
,
[Characterization of the gene encoding the polymorphic immunodominant molecule, a neutralizing antigen of Theileria parva]
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Characterization of the gene encoding the polymorphic immunodominant molecule, a neutralizing antigen of Theileria parva
Nene, Vishvanath
,
Iams, K.P.
,
Gobright, E.I.
,
Musoke, A.J.
,
[Characterisation of the gene encoding a candidate vaccine antigen of Theileria parva sporozoites]
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Characterisation of the gene encoding a candidate vaccine antigen of Theileria parva sporozoites
Previous data showed that six out of a group of nine cattle inoculated with NS1-p67, a recombinant form of a 67-KDa Theileria parva sporozoite surface protein, were immune to East Coast fever. This bacterialy expressed antigen encoded all 709 amino acid residues of p67 fused to the C-terminal end...
Nene, Vishvanath
,
Inumaru, S.
,
McKeever, Declan J.
,
Morzaria, S.P.
,
Shaw, M.
,
Musoke, A.J.
,
[Characterization of an insect cell-derived Theileria parva sporozoite vaccine antigen and immunogenicity in cattle]
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Characterization of an insect cell-derived Theileria parva sporozoite vaccine antigen and immunogenicity in cattle
Nyagwange, James
,
Awino, Elias
,
Tijhaar, Edwin
,
Svitek, Nicholas
,
Pelle, Roger
,
Nene, Vishvanath
,
[Leveraging the Medicines for Malaria Venture malaria and pathogen boxes to discover chemical inhibitors of East Coast fever]
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Leveraging the Medicines for Malaria Venture malaria and pathogen boxes to discover chemical inhibitors of East Coast fever