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Initial comparison of 2 polymorphic, immunodominant molecule (PIM) cDNA sequences from Theileria parva suggested that the PIM genes consist of conserved 5' and 3' termini flanking a central variable region. Further evidence is presented, based on sequence analysis, supporting this general structu...


Toye, Philip G.Gobright, E.I.Nyanjui, J.Nene, VishvanathBishop, Richard P.[Structure and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PlM), a candidate diagnostic antigen of Theileria parva]Structure and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PlM), a candidate diagnostic antigen of Theileria parva

We describe the generation of an auto-annotated index of genes that are expressed in the salivary glands of four-day fed female adult Rhipicephalus appendiculatus ticks. A total of 9162 EST sequences were derived from an uninfected tick cDNA library and 9844 ESTs were from a cDNA library from t...


Nene, VishvanathLee, D.Kang'a, S.Skilton, Robert A.Shah, TushaarVilliers, Etienne P. deMwaura, S.Taylor, D.Quackenbush, J.Bishop, Richard P.[Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva]Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva

Random clones from a cDNA library made from mRNA purified from dissected salivary glands of feeding female Amblyomma variegatum ticks were subjected to single pass sequence analysis. A total of 3,992 sequences with an average read length of 580 nucleotides have been used to construct a gene index...


Nene, VishvanathLee, D.Quackenbush, J.Skilton, Robert A.Mwaura, S.Gardner, M.J.Bishop, Richard P.[AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum]AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum

Protein components of the cement cone of ixodid ticks are candidates for inclusion in vaccines against tick infestation, since they are essential for tick attachment and feeding. We describe here the cloning of a cDNA encoding a 36 kDa protein, designated Rhipicephalus Immuno-dominant Molecule 36...


Bishop, Richard P.Lambson, B.Wells, C.Pandit, P.Osaso, J.Nkonge, C.Morzaria, S.P.Musoke, A.J.Nene, Vishvanath[A cement protein of the tick Rhipicephalus appendiculatus, located in the secretory e cell granules of the type III salivary gland acini, induces strong antibody responses in cattle]A cement protein of the tick Rhipicephalus appendiculatus, located in the secretory e cell granules of the type III salivary gland acini, induces strong antibody responses in cattle

Polymorphic, multicopy gene families are frequently located in subtelomeric regions of the genomes of parasitic protozoa. Theileria parva telomere-associated (TA) DNA from two chromosomes contained long open reading frames (ORFs) 54% identical at the N-termini whose ends were 2670 and 2680 bp fro...


Bishop, Richard P.Gobright, E.I.Nene, VishvanathMorzaria, S.P.Musoke, A.J.Sohanpal, B.K.[Polymorphic open reading frames encoding secretory proteins are located less than 3 kilobases from Theileria parva telomeres]Polymorphic open reading frames encoding secretory proteins are located less than 3 kilobases from Theileria parva telomeres

Theileria parva, a tick-transmitted protozoan parasite related to Plasmodium spp., causes the disease East Coast fever, an acute and usually fatal lymphoproliferative disorder of cattle in Africa. Previous studies using sera from cattle that have survived infection identified a polymorphic immuno...


Toye, Philip G.Metzelaar, M.J.Wijngaard, P.L.J.Nene, VishvanathIams, K.P.Roose, J.Nyanjui, J.K.Gobright, E.I.Musoke, A.J.Clevers, H.C.[Characterization of the gene encoding the polymorphic immunodominant molecule, a neutralizing antigen of Theileria parva]Characterization of the gene encoding the polymorphic immunodominant molecule, a neutralizing antigen of Theileria parva
Nene, VishvanathIams, K.P.Gobright, E.I.Musoke, A.J.[Characterisation of the gene encoding a candidate vaccine antigen of Theileria parva sporozoites]Characterisation of the gene encoding a candidate vaccine antigen of Theileria parva sporozoites
Kibe, M.K.Ole-MoiYoi, O.K.Nene, VishvanathKhan, B.Allsopp, B.A.Collins, N.E.Morzaria, S.P.Gobright, E.I.Bishop, B.P.[Evidence for two single copy units in Theileria parva ribosomal RNA genes]Evidence for two single copy units in Theileria parva ribosomal RNA genes
Iams, K.P.Young, J.R.Nene, VishvanathDesai, J.Webster, P.Ole-MoiYoi, O.K.Musoke, A.J.[Characterization of the gene encoding a 104 kilodalton microneme-rhoptry protein of Theileria parva]Characterization of the gene encoding a 104 kilodalton microneme-rhoptry protein of Theileria parva

Molecules located on cell surfaces and those that are secreted from the cell frequently play a critical role in cell biology by mediating interaction with the external environment. These types of parasite molecules often constitute targets for protective host immune responses and are thus of addi...


Musembi, S.Janoo, R.T.K.Sohanpal, B.K.Ochanda, H.Ole-MoiYoi, O.K.Bishop, Richard P.Nene, Vishvanath[Screening for Theileria parva secretory gene products by functional analysis in Saccharomyces cerevisiae]Screening for Theileria parva secretory gene products by functional analysis in Saccharomyces cerevisiae

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