Theileria are economically important, intra-cellular protozoa, transmitted by ixodid ticks, which infect wild and domestic ruminants. In the mammalian host, parasites infect leukocytes and erythrocytes. In the arthropod vector they develop in gut epithelial cells and salivary glands. All four int...
Bishop, Richard P.
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Musoke, A.J.
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Morzaria, S.P.
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Gardner, M.
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Nene, Vishvanath
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[Theileria: Intracellular protozoan parasites of wild and domestic ruminants transmitted by ixodid ticks]
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Theileria: Intracellular protozoan parasites of wild and domestic ruminants transmitted by ixodid ticks
When compared with other Apicomplexan organisms. Theileria parva has an execptionally small, 10-12 Mbp. genome. There are only 4 chromosomes, each in the Mbp range, and a complete physical map, based on SfiI linking data, is available for each one. A number of genes and cDNAs have been mapped to ...
Nene, Vishvanath
,
Morzaria, S.P.
,
Bishop, Richard P.
,
[Organisation and informational content of the Theileria parva genome]
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Organisation and informational content of the Theileria parva genome
Theileria are tick-transmitted, haemoprotozoan parasites infecting wild and domestic ungulates throughout many areas of the world. The most economically important species are T. parva and T. annulata which are pathogenic to cattle, and T. lestoquardi (syn hirci) which is pathogenic to sheep and g...
Skilton, Robert A.
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Musoke, A.J.
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Nene, Vishvanath
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Wasawo, D.P.S.
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Wells, C.W.
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Spooner, P.R.
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Bishop, Richard P.
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Osaso, J.
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Nkonge, C.
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Latif, A.
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Morzaria, S.P.
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[Molecular characterisation of a Theileria lestoquardi gene encoding a candidate sporozoite vaccine antigen]
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Molecular characterisation of a Theileria lestoquardi gene encoding a candidate sporozoite vaccine antigen
Theileria annulata and Theileria parva both poses a major surface antigen on the sporozoite stage of the life-cycle, called SPAG-1 and p67, respectively. In each case, these antigens are vaccine candidates and have been shown to induce a degree of homologous protection in earlier work. These anti...
Hall, R.
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Boulter, N.R.
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Brown, C.G.D.
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Wilkie, G.
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Kirvar, E.
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Nene, Vishvanath
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Musoke, A.J.
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Glass, E.J.
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Morzaria, S.P.
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[Reciprocal cross-protection induced by sporozoite antigens SPAG-1 from Theileria annulata and p67 from Theileria parva]
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Reciprocal cross-protection induced by sporozoite antigens SPAG-1 from Theileria annulata and p67 from Theileria parva
Full-length recombinant versions of p67, the 709 amino acid major surface protein of Theileria parva sporozoites, induce immunity to East Coast fever (ECF) in cattle. We show that a soluble Escherichia coli recombinant version of p67 (p67635), in which a prokaryotic signal peptide replaces the e...
Bishop, Richard P.
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Nene, Vishvanath
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Staeyert, J.
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Rowlands, G.J.
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Nyanjui, J.
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Osaso, J.
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Morzaria, S.P.
,
Musoke, A.J.
,
[Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites]
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Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites
Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67DeltaSS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and th...
Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one i...
Musoke, A.J.
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Rowlands, G.J.
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Nene, Vishvanath
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Nyanjui, J.
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Katende, J.
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Spooner, P.R.
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Mwaura, S.
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Odongo, D.
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Nkonge, C.
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Mbogo, S.
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Bishop, Richard P.
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Morzaria, S.P.
,
[Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge]
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Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge
A stage-specific surface antigen of Theileria parva, p67, is the basis for the development of an anti-sporozoite vaccine for the control of East Coast fever (ECF) in cattle. By Pepscan analysis with a series of overlapping synthetic p67 peptides, the antigen was shown to contain five distinct lin...
Nene, Vishvanath
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Gobright, E.I.
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Bishop, Richard P.
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Morzaria, S.P.
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Musoke, A.J.
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[Linear peptide specificity of bovine antibody responses to p67 of Theileria parva and sequence diversity of sporozoite-neutralising epitopes: Implications for a vaccine]
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Linear peptide specificity of bovine antibody responses to p67 of Theileria parva and sequence diversity of sporozoite-neutralising epitopes: Implications for a vaccine
Immunity to Theileria parva infection in cattle is often parasite stock specific. The antigenic diversity which is expressed at the schizont stage of the parasite together with a wild reservoir of the organism in buffalo has complicated the development of effective disease control by immunization...
Nene, Vishvanath
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Musoke, A.J.
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Gobright, E.I.
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Morzaria, S.P.
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[Conservation of the sporozoite p67 vaccine antigen in cattle-derived Theileria parva stocks with different cross-immunity profiles]
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Conservation of the sporozoite p67 vaccine antigen in cattle-derived Theileria parva stocks with different cross-immunity profiles