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Kairo, A.Gobright, E.I.Fairlamb, A.H.Nene, Vishvanath[A 7.1 Kb linear DNA molecule of Theileria parva has scrambled rDNA sequences and open reading frames for mitochondrially encoded proteins]A 7.1 Kb linear DNA molecule of Theileria parva has scrambled rDNA sequences and open reading frames for mitochondrially encoded proteins

Massively parallel signature sequencing (MPSS) was used to analyze the transcriptome of the intracellular protozoan Theileria parva. In total 1 095 000, 20 bp sequences representing 4371 different signatures were generated from T.parva schizonts. Reproducible signatures were identified within 73%...


Bishop, Richard P.Shah, TushaarPelle, RogerHoyle, D.Pearson, T.Haines, L.Brass, A.Hulme, H.Graham, S.P.Taracha, E.L.N.Lu, C.Hass, B.Wortman, J.White, O.Gardner, M.J.Nene, VishvanathVilliers, Etienne P. de[Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage]Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage

Initial comparison of 2 polymorphic, immunodominant molecule (PIM) cDNA sequences from Theileria parva suggested that the PIM genes consist of conserved 5' and 3' termini flanking a central variable region. Further evidence is presented, based on sequence analysis, supporting this general structu...


Toye, Philip G.Gobright, E.I.Nyanjui, J.Nene, VishvanathBishop, Richard P.[Structure and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PlM), a candidate diagnostic antigen of Theileria parva]Structure and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PlM), a candidate diagnostic antigen of Theileria parva

We describe the generation of an auto-annotated index of genes that are expressed in the salivary glands of four-day fed female adult Rhipicephalus appendiculatus ticks. A total of 9162 EST sequences were derived from an uninfected tick cDNA library and 9844 ESTs were from a cDNA library from t...


Nene, VishvanathLee, D.Kang'a, S.Skilton, Robert A.Shah, TushaarVilliers, Etienne P. deMwaura, S.Taylor, D.Quackenbush, J.Bishop, Richard P.[Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva]Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva
Bishop, Richard P.Sohanpal, B.K.Kariuki, D.Young, A.S.Nene, VishvanathBaylis, H.Allsopp, B.A.Spooner, P.R.Dolan, T.T.Morzaria, S.P.[Detection of a carrier state in Theileria parva infected cattle using the polymerase chain reaction]Detection of a carrier state in Theileria parva infected cattle using the polymerase chain reaction

Polymorphic, multicopy gene families are frequently located in subtelomeric regions of the genomes of parasitic protozoa. Theileria parva telomere-associated (TA) DNA from two chromosomes contained long open reading frames (ORFs) 54% identical at the N-termini whose ends were 2670 and 2680 bp fro...


Bishop, Richard P.Gobright, E.I.Nene, VishvanathMorzaria, S.P.Musoke, A.J.Sohanpal, B.K.[Polymorphic open reading frames encoding secretory proteins are located less than 3 kilobases from Theileria parva telomeres]Polymorphic open reading frames encoding secretory proteins are located less than 3 kilobases from Theileria parva telomeres

Theileria are economically important, intra-cellular protozoa, transmitted by ixodid ticks, which infect wild and domestic ruminants. In the mammalian host, parasites infect leukocytes and erythrocytes. In the arthropod vector they develop in gut epithelial cells and salivary glands. All four int...


Bishop, Richard P.Musoke, A.J.Morzaria, S.P.Gardner, M.Nene, Vishvanath[Theileria: Intracellular protozoan parasites of wild and domestic ruminants transmitted by ixodid ticks]Theileria: Intracellular protozoan parasites of wild and domestic ruminants transmitted by ixodid ticks

When compared with other Apicomplexan organisms. Theileria parva has an execptionally small, 10-12 Mbp. genome. There are only 4 chromosomes, each in the Mbp range, and a complete physical map, based on SfiI linking data, is available for each one. A number of genes and cDNAs have been mapped to ...


Nene, VishvanathMorzaria, S.P.Bishop, Richard P.[Organisation and informational content of the Theileria parva genome]Organisation and informational content of the Theileria parva genome

Theileria annulata and Theileria parva both poses a major surface antigen on the sporozoite stage of the life-cycle, called SPAG-1 and p67, respectively. In each case, these antigens are vaccine candidates and have been shown to induce a degree of homologous protection in earlier work. These anti...


Hall, R.Boulter, N.R.Brown, C.G.D.Wilkie, G.Kirvar, E.Nene, VishvanathMusoke, A.J.Glass, E.J.Morzaria, S.P.[Reciprocal cross-protection induced by sporozoite antigens SPAG-1 from Theileria annulata and p67 from Theileria parva]Reciprocal cross-protection induced by sporozoite antigens SPAG-1 from Theileria annulata and p67 from Theileria parva

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