Protein components of the cement cone of ixodid ticks are candidates for inclusion in vaccines against tick infestation, since they are essential for tick attachment and feeding. We describe here the cloning of a cDNA encoding a 36 kDa protein, designated Rhipicephalus Immuno-dominant Molecule 36...
Bishop, Richard P.
,
Lambson, B.
,
Wells, C.
,
Pandit, P.
,
Osaso, J.
,
Nkonge, C.
,
Morzaria, S.P.
,
Musoke, A.J.
,
Nene, Vishvanath
,
[A cement protein of the tick Rhipicephalus appendiculatus, located in the secretory e cell granules of the type III salivary gland acini, induces strong antibody responses in cattle]
,
A cement protein of the tick Rhipicephalus appendiculatus, located in the secretory e cell granules of the type III salivary gland acini, induces strong antibody responses in cattle
Theileria are tick-transmitted, haemoprotozoan parasites infecting wild and domestic ungulates throughout many areas of the world. The most economically important species are T. parva and T. annulata which are pathogenic to cattle, and T. lestoquardi (syn hirci) which is pathogenic to sheep and g...
Skilton, Robert A.
,
Musoke, A.J.
,
Nene, Vishvanath
,
Wasawo, D.P.S.
,
Wells, C.W.
,
Spooner, P.R.
,
Bishop, Richard P.
,
Osaso, J.
,
Nkonge, C.
,
Latif, A.
,
Morzaria, S.P.
,
[Molecular characterisation of a Theileria lestoquardi gene encoding a candidate sporozoite vaccine antigen]
,
Molecular characterisation of a Theileria lestoquardi gene encoding a candidate sporozoite vaccine antigen
Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p15...
Katende, J.
,
Morzaria, S.P.
,
Toye, Philip G.
,
Skilton, Robert A.
,
Nene, Vishvanath
,
Nkonge, C.
,
Musoke, A.J.
,
[An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule]
,
An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule
Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one i...
Musoke, A.J.
,
Rowlands, G.J.
,
Nene, Vishvanath
,
Nyanjui, J.
,
Katende, J.
,
Spooner, P.R.
,
Mwaura, S.
,
Odongo, D.
,
Nkonge, C.
,
Mbogo, S.
,
Bishop, Richard P.
,
Morzaria, S.P.
,
[Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge]
,
Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge
Rurangirwa, F.R.
,
Musoke, A.J.
,
Nantulya, V.M.
,
Nkonge, C.
,
Njuguna, L.
,
Mushi, E.Z.
,
Karstad, L.
,
Grootenhuis, J.G.
,
[Immune effector mechanisms involved in the control of parasitaemia in Trypanosoma brucei-infected wildebeest (Connochaetes taurinus)]
,
Immune effector mechanisms involved in the control of parasitaemia in Trypanosoma brucei-infected wildebeest (Connochaetes taurinus)
To identify the genes encoding novel immunodominant antigens of Theileria parvo a ?gt11 library of piroplasm genomic DNA was immunoscreened with bovine recovery serum and a gene encoding a 150 kDa antigen (p150) was identified. The predicted polypeptide contains an N-terminal secretory signal seq...
Skilton, Robert A.
,
Bishop, Richard P.
,
Wells, C.W.
,
Spooner, P.R.
,
Gobright, E.I.
,
Nkonge, C.
,
Musoke, A.J.
,
Macklin, M.D.
,
Iams, K.P.
,
[Cloning and characterization of a 150 kDa microsphere antigen of Theileria parva that is immunologically cross-reactive with the polymorphic immunodominant molecule (PIM)]
,
Cloning and characterization of a 150 kDa microsphere antigen of Theileria parva that is immunologically cross-reactive with the polymorphic immunodominant molecule (PIM)
The Development of a recombinant Theileria parva vaccine is discussed under the following headings: mechanisms of protection against ECF [East Coast fever]; cloning and expression of p67; immunization and challenge of cattle; interpretation of immunization and challenge data.
Musoke, A.J.
,
McKeever, Declan J.
,
Morzaria, S.P.
,
Nkonge, C.
,
Nene, Vishvanath
,
[A recombinant vaccine for Theileria parva]
,
A recombinant vaccine for Theileria parva
Musoke, A.J.
,
Morzaria, S.P.
,
Nkonge, C.
,
Jones, E.
,
Nene, Vishvanath
,
[A recombinant sporozoite surface antigen of Theileria parva induces protection in cattle]
,
A recombinant sporozoite surface antigen of Theileria parva induces protection in cattle
Theileria parva, a protozoan parasite of cattle, causes a disease called East Coast fever. The disease is of economic importance because it causes high morbidity and mortality and limits the development of the cattle industry. This paper highlights the experiences with immunization against Theile...
Musoke, A.J.
,
McKeever, Declan J.
,
Morzaria, S.P.
,
Nkonge, C.
,
Nene, Vishvanath
,
[Experiences with immunization against Theileria parva using p67, a sporozoite surface antigen]
,
Experiences with immunization against Theileria parva using p67, a sporozoite surface antigen