Microarrays are an important and widely used tool. Applications include capturing genomic DNA for high-throughput sequencing in addition to the traditional monitoring of gene expression and identifying DNA copy number variations. Sequence mismatches between probe and target strands are known to a...
Rennie, C.
,
Noyes, H.A.
,
Kemp, Stephen J.
,
Hulme, H.
,
Brass, A.
,
Hoyle, D.C.
,
[Strong position-dependent effects of sequence mismatches on signal ratios measured using long oligonucleotide microarrays]
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Strong position-dependent effects of sequence mismatches on signal ratios measured using long oligonucleotide microarrays
High-through put technologies inevitably produce vast quantities of data. This presents challenges in terms of developing effective analysis methods, particularly where the analysis involves combining data derived from different experimental technologies. In this investigation, a systematic appro...
Rennie, C.
,
Hulme, H.
,
Fisher, P.
,
Halp, L.
,
Agaba, Morris
,
Noyes, H.A.
,
Kemp, Stephen J.
,
Brass, A.
,
[A systematic, data-driven approach to the combined analysis of microarray and QTL data]
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A systematic, data-driven approach to the combined analysis of microarray and QTL data
Trypanosoma congolense is a protozoan parasite that causes severe diseases in livestock. Three major quantative trait loci (QTL), Tir1, Tir2, and Tir3, control the survival time of mice after infection with T. congolense. Congenic mice carrying the C57BL/6 resistance alleles on the A/J background...
Rathkolb, B.
,
Noyes, H.A.
,
Brass, A.
,
Dark, P.
,
Fuchs, H.
,
Gailus-Durner, V.
,
Gibson, John P.
,
Angelis, M.H. de
,
Ogugo, M.
,
Iraqi, F.A.
,
Kemp, Stephen J.
,
Naessens, Jan
,
Pope, M.E.
,
Wolf, E.
,
Agaba, Morris
,
[Clinical chemistry of congenic mice with quantitative trait loci for predicted responses to Trypanosoma congolense infection]
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Clinical chemistry of congenic mice with quantitative trait loci for predicted responses to Trypanosoma congolense infection
Trypanosoma congolense are extracellular protozoan parasites of the blood stream of artiodactyls and are one of the main constraints on cattle production in Africa. In cattle, anaemia is the key feature of disease and persists after parasitaemia has declined to low or undetectable levels, but tre...
Noyes, H.A.
,
Alimohammadian, M.H.
,
Agaba, Morris
,
Brass, A.
,
Fuchs, H.
,
Gailus-Durner, V.
,
Hulme, H.
,
Iraqi, F.A.
,
Kemp, Stephen J.
,
Rathkolb, B.
,
Wolf, E.
,
Angelis, M.H. de
,
Roshandel, D.
,
Naessens, Jan
,
[Mechanisms controlling anaemia in Trypanosoma congolense infected mice]
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Mechanisms controlling anaemia in Trypanosoma congolense infected mice
Differences in gene expression may be caused by nearby DNA polymorphisms (cis regulation) or by interactions of gene control regions with polymorphic transcription factors (trans regulation). Trans acting loci are much harder to detect than cis acting loci and their effects are much more sensitiv...
Noyes, H.A.
,
Agaba, Morris
,
Anderson, S.
,
Archibald, A.L.
,
Brass, A.
,
Gibson, John P.
,
Hall, L.
,
Hulme, H.
,
Oh, S.J.
,
Kemp, Stephen J.
,
[Genotype and expression analysis of two inbred mouse strains and two derived congenic strains suggest that most gene expression is trans regulated and sensitive to genetic background]
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Genotype and expression analysis of two inbred mouse strains and two derived congenic strains suggest that most gene expression is trans regulated and sensitive to genetic background
An international multidisciplinary consortium is conducting a programme of research on the host response to trypanosome infection. This builds upon quantitative trait loci (QTL) mapping which identified genome regions influencing susceptibility to pathology following T. congolense infection in bo...
Kemp, Stephen J.
,
Hanotte, Olivier H.
,
Agaba, Morris
,
Noyes, H.A.
,
Gibson, John P.
,
Archibald, A.L.
,
Rennie, C.
,
Hulme, H.
,
Brass, A.
,
[Genomics approaches to study the biology underlying resistance to trypanosomiasis--some unexpected lessons]
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Genomics approaches to study the biology underlying resistance to trypanosomiasis--some unexpected lessons
African trypanosomes are protozoan parasites that cause “sleeping sickness” in humans and a similar disease in livestock. Trypanosomes also infect laboratory mice and three major quantitative trait loci (QTL) that regulate survival time after infection with T. congolense have been identified in t...
Goodhead, I.
,
Archibald, A.L.
,
Amwayi, P.
,
Brass, A.
,
Gibson, John P.
,
Hall, N.
,
Hughes, Monica A.
,
Limo, M.K.H.
,
Iraqi, F.A.
,
Kemp, Stephen J.
,
Noyes, H.A.
,
[A comprehensive genetic analysis of candidate genes regulating response to Trypanosoma congolense infection in mice]
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A comprehensive genetic analysis of candidate genes regulating response to Trypanosoma congolense infection in mice
We review the global dynamics of livestock disease over the last two decades. Our imperfect ability to detect and report disease hinders assessment of trends, but we suggest that, although endemic diseases continue their historic decline in wealthy countries, poor countries experience static or d...
Noyes, H.A.
,
Brass, A.
,
Obara, I.
,
Anderson, S.I.
,
Archibald, A.L.
,
Bradley, D.G.
,
Fisher, P.
,
Freeman, A.R.
,
Gibson, John P.
,
Gicheru, M.
,
Hall, L.
,
Hanotte, Olivier H.
,
Hulme, H.
,
McKeever, Declan J.
,
Murray, C.
,
Oh, S.J.
,
Tate, C.
,
Smith, K.C.
,
Tapio, M.
,
Wambugu, J.
,
Williams, D.
,
Agaba, Morris
,
Kemp, Stephen J.
,
[Genetic and expression analysis of cattle identifies candidate genes in pathways responding to Trypanosoma congolense infection]
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Genetic and expression analysis of cattle identifies candidate genes in pathways responding to Trypanosoma congolense infection