Inadequate nutrition is the main cause of low productivity of livestock in sub-Saharan Africa. The primary feed resources include fodder trees, natural pastures and crop residues. This paper presents results from studies conducted at ILRI in evaluations of these resources. The effect of supplemen...
Osuji, P.O.
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Fernández Rivera, S.
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Odenyo, A.A.
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[Improving fibre utilisation and protein supply in animals fed poor quality roughages: ILRI nutrition research and plans]
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Improving fibre utilisation and protein supply in animals fed poor quality roughages: ILRI nutrition research and plans
Three strains of tannin-tolerant rumen bacteria were isolated from enrichment cultures of rumen microflora of sheep, goat, and antelope and established in medium containing high concentrations of crude tannin extract or tannic acid. These three strains (EAT2, ES3, and EG19), characterized as Sele...
The aim of this study was to isolate and characterize bacteria capable of hydrolysing tannins from the faeces of Ethiopian ruminants adapted to feed on tannin-rich leaves. Faecal samples were collected from dikdik (Madoqua guentheri), camel (Camelus dromedaries), Grant's gazelle (Gazella granti),...
Ephraim, E.
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Odenyo, A.A.
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Ashenafi, M.
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[Isolation and characterization of tannin-degrading bacteria from faecal samples of some wild ruminants in Ethiopia]
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Isolation and characterization of tannin-degrading bacteria from faecal samples of some wild ruminants in Ethiopia
Fermentation of legume fodder tree leaves by rumen microorganisms was evaluated. The substrates were sun-dried, ground leaves. Gas and volatile fatty acid (VFAs) production were estimated. Using gas production as an index of fermentation at 12 h, the leaves tested ranked as follows; Chamaecytisus...
Odenyo, A.A.
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Osuji, P.O.
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Negassa, D.
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[Microbial evaluation of fodder tree leaves as ruminant feed]
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Microbial evaluation of fodder tree leaves as ruminant feed
Acacia angustissima has potential as a fodder tree, but was toxic to sheep when fed without adaptation at levels higher than 50 g per day in previous studies. In the present study it was determined that rats are sensitive to anti-nutritional factor(s) of A. angustissima and can therefore be used ...
Smith, A.H.
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Odenyo, A.A.
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Osuji, P.O.
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Wallig, M.A.
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Kandil, F.E.
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Seigler, D.S.
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Mackie, R.I.
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[Evaluation of toxicity of Acacia angustissima in a rat bioassay]
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Evaluation of toxicity of Acacia angustissima in a rat bioassay
This work examined the ability of microorganisms from African ungulates to degrade 4-N-acetyl-2,4-diaminobutyric acid (ADAB) found in A. angustissima leaves, as well as purified ADAB and diaminobutyric acid (DABA). These microorganisms were taken from the rumens of bush duiker, eland, impala, Gra...
There are conflicting Reports in the literature about the benefits of defaunation, the removal of ciliate protozoa from the rumen, in ruminant production. Ciliate protozoa are not essential for proper functioning of the rumen nor for the life of the host and that their contribution to microbial p...
Teferedegne, B.
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Osuji, P.O.
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Odenyo, A.A.
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Wallace, R.J.
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Newbold, C.J.
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[Effect of defaunation on utilisation of poor quality tropical feed by sheep]
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Effect of defaunation on utilisation of poor quality tropical feed by sheep
Foliage from the tropical leguminous tree, Sesbania sesban, is toxic to rumen protozoa in vitro, due to materials present in a saponins-containing extract of the foliage (Newbold et al 1997). Suppression of protozoal numbers in vivo when S. sesban is added to the diet is either transient or non-e...
Teferedegne, B.
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Osuji, P.O.
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Odenyo, A.A.
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Wallace, R.J.
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Newbold, C.J.
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[Influence of saponins and sapogenins on the bacteriolytic activity of ciliate protozoa from the sheep rumen]
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Influence of saponins and sapogenins on the bacteriolytic activity of ciliate protozoa from the sheep rumen
Sequences from a tannin-tolerant Selenomonas ruminantium isolate (EAT2) that hydrolyzes gallic acid were identified. Two exhibited identity to helicases with a wide phylogenetic distribution. PCR amplification by using primers from one helicase gene detected 2,000 to 5,000 EAT2 genome equivalents...
Bishop, Richard P.
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Obura, M.
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Odongo, D.
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Odenyo, A.A.
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[Specific PCR assay for a tannin-tolerant Selenomonas ruminantium isolate, derived from helicase coding sequences]
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Specific PCR assay for a tannin-tolerant Selenomonas ruminantium isolate, derived from helicase coding sequences