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Salp15, a 15-kDa tick salivary gland protein, has several suppressive modes of activity against host immunity and plays a critical role in the transmission of Lyme disease spirochetes in Ixodes scapularis and Ixodes ricinus, major vectors of Lyme disease in North America and Western Europe. Salp1...


Murase, Y.Konnai, S.Yamada, S.Githaka, Naftaly W.Isezaki, M.Ito, T.Takano, A.Ando, S.Kawabata, H.Murata, S.Ohashi, K.[An investigation of binding ability of Ixodes persulcatus Schulze Salp15 with Lyme disease spirochetes]An investigation of binding ability of Ixodes persulcatus Schulze Salp15 with Lyme disease spirochetes

Ixodes ricinus immunosuppressor (Iris) is a tick salivary gland protein derived from I. ricinus. In this study, Iris homolog was identified in the salivary glands of Ixodes persulcatus, which is the specific vector of the Lyme disease agent in Japan. The homolog was named Ipis-1. To investigate t...


Toyomane, K.Konnai, S.Niwa, A.Githaka, Naftaly W.Isezaki, M.Yamada, S.Ito, T.Takano, A.Ando, S.Kawabata, H.Murata, S.Ohashi, K.[Identification and the preliminary in vitro characterization of IRIS homologue from salivary glands of Ixodes persulcatus Schulze]Identification and the preliminary in vitro characterization of IRIS homologue from salivary glands of Ixodes persulcatus Schulze

In this work we evaluated several genes involved in gluconeogenesis, glycolysis and glycogen metabolism, the major pathways for carbohydrate catabolism and anabolism, in the BME26 Rhipicephalus microplus embryonic cell line. Genetic and catalytic control of the genes and enzymes associated with t...


Silva, Robson Marinho daNoce, B.D.Waltero, C.F.Costa, E.P.Abreu, L.A. deGithaka, Naftaly W.Moraes, J.Gomes, H.F.Konnai, S.Ida, V.S. jrOhashi, K.Logullo, C.[Non-classical gluconeogenesis-dependent glucose metabolism in Rhipicephalus microplus embryonic cell line BME26]Non-classical gluconeogenesis-dependent glucose metabolism in Rhipicephalus microplus embryonic cell line BME26

Piroplasms frequently infect domestic and wild carnivores. At present, there is limited information on the occurrence and molecular identity of these tick-borne parasites in wild felids in Kenya. In 2009, a pair of captive lions (Panthare leo) was diagnosed with suspected babesiosis and mineral d...


Githaka, Naftaly W.Konnai, S.Kariuki, E.Kanduma, E.G.Murata, S.Ohashi, K.[Molecular detection and characterisation of potentially new Babesia and Theileria species/variants in wild felids from Kenya]Molecular detection and characterisation of potentially new Babesia and Theileria species/variants in wild felids from Kenya

Recently, mortalities among giraffes, attributed to infection with unique species of piroplasms were reported in South Africa. Although haemoparasites are known to occur in giraffes of Kenya, the prevalence, genetic diversity and pathogenicity of these parasites has not been investigated. In thi...


Githaka, Naftaly W.Konnai, S.Skilton, Robert A.Kariuki, E.Kanduma, E.G.Murata, S.Ohashi, K.[Genotypic variations in field isolates of Theileria species infecting giraffes (Giraffa camelopardalis tippelskirchi and Giraffa camelopardalis reticulata) in Kenya]Genotypic variations in field isolates of Theileria species infecting giraffes (Giraffa camelopardalis tippelskirchi and Giraffa camelopardalis reticulata) in Kenya

Inorganic PPases are essential metal-dependent enzymes that convert pyrophosphate into orthophosphate. This reaction is quite exergonic and provides a thermodynamic advantage for many ATP-driven biosynthetic reactions. We have previously demonstrated that cytosolic PPase from R. microplus embryos...


Costa, E.P.Façanha, A.R.Cruz, C.S.Silva, J.N.Machado, J.A.Carvalho, G.M.Fernandes, M.R.Martins, R.Campos, E.Romeiro, N.C.Githaka, Naftaly W.Konnai, S.Ohashi, K.Vaz, I.S.Logullo, C.[A novel mechanism of functional cooperativity regulation by thiol redox status in a dimeric inorganic pyrophosphatase]A novel mechanism of functional cooperativity regulation by thiol redox status in a dimeric inorganic pyrophosphatase

Among arthropods, ticks lead as vectors of animal diseases and rank second to mosquitoes in transmitting human pathogens. Cyclin-dependent kinases (CDK) participate in cell cycle control in eukaryotes. CDKs are serine/threonine protein kinases and these catalytic subunits are activated or inactiv...


Gomes, H.Moraes, J.Githaka, Naftaly W.Martins, R.Isezaki, M.Silva Vaz, I. daLogullo, C.Konnai, S.Ohashi, K.[Vaccination with cyclin-dependent kinase tick antigen confers protection against Ixodes infestation]Vaccination with cyclin-dependent kinase tick antigen confers protection against Ixodes infestation

The use of tick vaccines in mammalian hosts has been shown to be the most promising alternative tick control method to current use of acaricides, which suffers from a number of limitations. However, the success of this method is dependent on the identification cloning, and in vitro expression of ...


Mulenga, A.Sugimoto, C.Sako, C.Ohashi, K.Musoke, A.J.Shubash, M.Onuma, M.[Molecular characterization of Haemaphysalis longicornis tick salivary gland-associated 29-kilodalton protein and its effect as a vaccine against tick infestation in rabbits]Molecular characterization of Haemaphysalis longicornis tick salivary gland-associated 29-kilodalton protein and its effect as a vaccine against tick infestation in rabbits

Githaka, Naftaly W.Konnai, S.Bishop, Richard P.Odongo, D.Lekolool, I.Kariuki, E.Gakuya, F.Kamau, L.Isezaki, M.Murata, S.Ohashi, K.[Identification and sequence characterization of novel Theileria genotypes from the waterbuck (Kobus defassa) in a Theileria parva-endemic area in Kenya]Identification and sequence characterization of novel Theileria genotypes from the waterbuck (Kobus defassa) in a Theileria parva-endemic area in Kenya

The vitellogenin receptor (VgR), which belongs to the low-density lipoprotein receptors(LDLR) family, regulates the absorption of yolk protein accumulated in developing oocytes during oogenesis. In the present study, the full sequence of Rhipicephalus microplus VgR (RmVgR) and the partial sequenc...


Seixas, A.Alzugaray, M.F.Tirloni, T.Parizi, L.F.Pinto, A.F.M.Githaka, Naftaly W.Konnai, S.Ohashi, K.Yates, J.R.Termignoni, C.Silva Vaz, I. da[Expression profile of Rhipicephalus microplus vitellogenin receptor during oogenesis]Expression profile of Rhipicephalus microplus vitellogenin receptor during oogenesis

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