The biological effects of affinity purified seed lectin from African yam bean, Sphenostylis stenocarpa (Harms), were tested on Clavigralla tomentosicollis (Stål) in an artificial seed system. S. stenocarpa was extracted from two African yam bean accessions: Enugu 95-3 and Enugu 98-2. Lectins from...
The cowpea beetle, Callosobruchus maculatus, and pod-sucking bug, Clavigralla tomentosicollis, are two of the major insect pests of cowpea in Africa. A lectin was purified from the seeds of the African yam bean (AYB), Sphenostylis stenocarpa, by affinity chromatography on Galactosc-Sepharose 4B. ...
Okeola, O.G.
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Machuka, J.
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Fasidi, I.O.
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[Insecticidal activities of the African yam bean seed lectin on the development of the cowpea beetle and the pod sucking bug]
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Insecticidal activities of the African yam bean seed lectin on the development of the cowpea beetle and the pod sucking bug
A new galactose-specific lectin was isolated from African yam bean (Sphenostyles stenocarpa Harms) by affinity chromatography on galactose-Sepharose 4B. SDS–PAGE analysis resulted in four polypeptide bands of approximately 27, 29, 32 and 34 kDa, respectively. Based on the analysis of carbohydrate...