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Aims: To develop a multiplex TaqMan-based real-time PCR assay (qPCR) for the simultaneous detection and quantification of both RNA and DNA viruses affecting cassava (Manihot esculenta) in eastern Africa. Methods and Results: The diagnostic assay was developed for two RNA viruses; Cassava brown st...


Otti, G.Bouvaine, S.Kimata, B.Mkamillo, G.Kumar, P.L.Tomlins, Keith I.Maruthi, M.N.[High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants]High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants

In a bid to fast-track the process of selection of cassava (Manihot esculenta Crantz) genotypes resistant to the cassava mosaic disease (CMD), caused by whitefly-transmitted begomoviruses, a number of molecular markers have been developed to map resistance genes. The simple sequence repeat (SSR) ...


Gedil, M.Otti, G.Enok, L.Kumar, L.[Development and validation of genomic tools for marker-assisted breeding of cassava mosaic disease resistance in cassava (Manihot esculenta)]Development and validation of genomic tools for marker-assisted breeding of cassava mosaic disease resistance in cassava (Manihot esculenta)

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