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Cross contamination arising from random natural processes and breeding errors is one of the prominent challenges to breeding in many crops including cassava (Manihot esculenta). This study attempts to identify molecular marker tools suitable for verifying the genetic purity of putative progenies ...


Otti, G.Fakoya, A.Andrew, I.Gedil, M.[Development of genomic tools for verification of hybrids and selfed progenies in cassava (Manihot esculenta)]Development of genomic tools for verification of hybrids and selfed progenies in cassava (Manihot esculenta)

Aims: To develop a multiplex TaqMan-based real-time PCR assay (qPCR) for the simultaneous detection and quantification of both RNA and DNA viruses affecting cassava (Manihot esculenta) in eastern Africa. Methods and Results: The diagnostic assay was developed for two RNA viruses; Cassava brown st...


Otti, G.Bouvaine, S.Kimata, B.Mkamillo, G.Kumar, P.L.Tomlins, Keith I.Maruthi, M.N.[High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants]High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants

Here, we report a method to clean cassava plants from viral infections that cause cassava mosaic and brown streak diseases in Africa. Infected plants of resistant or tolerant varieties from Malawi, Mozambique, Kenya, Tanzania and Uganda were cleaned in the UK using a combination of tissue culture...


Maruthi, M.N.Whitfield, E.C.Otti, G.Tumwegamire, SilverKanju, E.Legg, J.P.Mkamilo, G.Kawuki, R.Benesi, I.Zacarias, A.Munga, T.Mwatuni, F.Mbugua, E.[A method for generating virus-free cassava plants to combat viral disease epidemics in Africa]A method for generating virus-free cassava plants to combat viral disease epidemics in Africa

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