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The impact of cassava transformation technologies for agricultural development in Africa will depend largely on how successfully these capabilities are transferred and adapted to the African environment and local needs. Here we report on the first successful establishment of cassava regeneration ...


Hankoua, B.Taylor, N.Ng, S.Fawole, I.Puonti-Kaerlas, J.Padmanabhan, C.Yadav, J.S.Fauquet, Claude M.Dixon, A.Fondong, V.N.[Production of the first transgenic cassava in Africa via direct shoot organogenesis from friable embryogenic calli and germination of maturing somatic embryos]Production of the first transgenic cassava in Africa via direct shoot organogenesis from friable embryogenic calli and germination of maturing somatic embryos

Accurate detection of viruses in plants and animals is critical for agriculture production and human health. Deep sequencing and assembly of virus-derived small interfering RNAs has proven to be a highly efficient approach for virus discovery. Here we present VirusDetect, a bioinformatics pipelin...


Yi ZhengShan GaoPadmanabhan, C.Li, R.Galvez, M.Gutiérrez, D.Fuentes, S.Kai-Shu LingKreuze, J.F.Zhangjun Fei[VirusDetect: an automated pipeline for efficient virus discovery using deep sequencing of small RNAs.]VirusDetect: an automated pipeline for efficient virus discovery using deep sequencing of small RNAs.

Small interfering RNA (siRNA) duplexes are short (usually 21 to 24 bp) double-stranded RNAs (dsRNAs) with several overhanging nucleotides at both 5'- and 3'-ends. It has been found that siRNA duplexes bind the RNA-induced silencing complex (RISC) and cleave the sense strands with endonucleases. I...


Niu, X.Sun, Y.Chen, Z.Li, R.Padmanabhan, C.Ruan, J.Kreuze, J.F.Ling, K.S.Fei, Z.J.Gao, S.[Using small RNA-seq data to detect siRNA duplexes induced by plant viruses.]Using small RNA-seq data to detect siRNA duplexes induced by plant viruses.

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