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In order to introduce currently-available genes with agronomical value into banana, two genetic transformation protocols have been optimized. Firstly, regenerable protoplasts isolated from embryogenic cell suspensions of the cultivar Bluggoe have been used for the introduction of several chimaeri...
The term banana is meant throughout this chapter to cover dessert and cooking bananas as well as plantains. With an estimated total production of more than 76 million tons in 1992 (FAO 1993), banana is the no. 1 fruit crop in the world. Ninety-nine percent of world's annual production is cultivat...
Banana and plantain (Musa spp.) are the fourth largest food commodity after rice, wheat, and milk in terms of the gross value of production (CGIAR 1992). They are produced in the humid tropics of many developing economies; about 74 million tons are produced annually (FAO 1991).
We have developed a simple protocol to allow the production of transgenic banana plants. Foreign genes were delivered into embryogenic suspension cells using accelerated particles coated with DNA. Bombardment parameters were optimized for a modified particle gun resulting in high levels of transi...
Electroporation conditions were established for transient expression of introduced DNA in banana (Musa spp., cv. 'Bluggoe') protoplasts isolated from regenerable embryogenic cell suspensions. The following parameters were found to be highly influential: electroporation buffer, polyethylene glycol...
Bananas are monocots and members of the genus Musa, family Musaceae. They are the second largest fruit crop in the world with an annual production of about 65 million tons (INIBAP 1993). The export trade, involving just dessert bananas of the Cavendish subgroup, comprises only 10% of the total ba...
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