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We have developed a simple protocol to allow the production of transgenic banana plants. Foreign genes were delivered into embryogenic suspension cells using accelerated particles coated with DNA. Bombardment parameters were optimized for a modified particle gun resulting in high levels of transi...


Sági, L.Panis, B.Remy, S.Schoofs, H.Smet, K. deSwennen, R.Cammue, B.P.[Genetic transformation of banana and plantain (Musa spp.) via particle bombardment]Genetic transformation of banana and plantain (Musa spp.) via particle bombardment

Electroporation conditions were established for transient expression of introduced DNA in banana (Musa spp., cv. 'Bluggoe') protoplasts isolated from regenerable embryogenic cell suspensions. The following parameters were found to be highly influential: electroporation buffer, polyethylene glycol...


Sagi, L.Remy, S.Panis, B.Swennen, R.Volckaert, G.[Transient gene expression in electroporated banana (Musa spp., cv. Bluggoe, ABB group) protoplasts isolated from regenerable embryogenic cell suspensions]Transient gene expression in electroporated banana (Musa spp., cv. Bluggoe, ABB group) protoplasts isolated from regenerable embryogenic cell suspensions

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