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Bananas are a staple food in Eastern Africa, with 25.3% of the total world production. The production is, however, threatened by the presence of several diseases, of which the fungal diseases black sigatoka and Panama disease are the most important. With the development of embryogenic cell suspen...


Smet, K. dePanis, B.Sagi, L.Cammue, B.Swennen, R.[Improvement of bananas for black sigatoka and Panama disease resistance through genetic manipulation]Improvement of bananas for black sigatoka and Panama disease resistance through genetic manipulation

In order to introduce currently-available genes with agronomical value into banana, two genetic transformation protocols have been optimized. Firstly, regenerable protoplasts isolated from embryogenic cell suspensions of the cultivar Bluggoe have been used for the introduction of several chimaeri...


Sagi, L.Remy, S.Verelst, B.Panis, B.Cammue, B.P.Volckaert, G.Swennen, R.[Transient gene expression in transformed banana (Musa cv. Bluggoe) protoplasts and embryogenic cell suspensions]Transient gene expression in transformed banana (Musa cv. Bluggoe) protoplasts and embryogenic cell suspensions

The term banana is meant throughout this chapter to cover dessert and cooking bananas as well as plantains. With an estimated total production of more than 76 million tons in 1992 (FAO 1993), banana is the no. 1 fruit crop in the world. Ninety-nine percent of world's annual production is cultivat...


Sagi, L.Remy, S.Verelst, B.Swennen, R.Panis, B.[Genetic transformation in Musa species (Banana)]Genetic transformation in Musa species (Banana)

Banana and plantain (Musa spp.) are the fourth largest food commodity after rice, wheat, and milk in terms of the gross value of production (CGIAR 1992). They are produced in the humid tropics of many developing economies; about 74 million tons are produced annually (FAO 1991).


Panis, B.Swennen, R.[Cryopreservation of germplasm of banana and plantain]Cryopreservation of germplasm of banana and plantain

We have developed a simple protocol to allow the production of transgenic banana plants. Foreign genes were delivered into embryogenic suspension cells using accelerated particles coated with DNA. Bombardment parameters were optimized for a modified particle gun resulting in high levels of transi...


Sági, L.Panis, B.Remy, S.Schoofs, H.Smet, K. deSwennen, R.Cammue, B.P.[Genetic transformation of banana and plantain (Musa spp.) via particle bombardment]Genetic transformation of banana and plantain (Musa spp.) via particle bombardment

Electroporation conditions were established for transient expression of introduced DNA in banana (Musa spp., cv. 'Bluggoe') protoplasts isolated from regenerable embryogenic cell suspensions. The following parameters were found to be highly influential: electroporation buffer, polyethylene glycol...


Sagi, L.Remy, S.Panis, B.Swennen, R.Volckaert, G.[Transient gene expression in electroporated banana (Musa spp., cv. Bluggoe, ABB group) protoplasts isolated from regenerable embryogenic cell suspensions]Transient gene expression in electroporated banana (Musa spp., cv. Bluggoe, ABB group) protoplasts isolated from regenerable embryogenic cell suspensions

Bananas are monocots and members of the genus Musa, family Musaceae. They are the second largest fruit crop in the world with an annual production of about 65 million tons (INIBAP 1993). The export trade, involving just dessert bananas of the Cavendish subgroup, comprises only 10% of the total ba...


Panis, B.Sagi, L.Swennen, R.[Regeneration of plants from protoplasts of Musa species (banana)]Regeneration of plants from protoplasts of Musa species (banana)

Plasma membrane proteins constitute a very important class of proteins. They are involved in the transmission of external signals to the interior of the cell and selective transport of water, nutrients and ions across the plasma membrane. However, the study of plasma membrane proteins is challeng...


García, S.Panis, B.Swennen, R.L.Carpentier, S.[Evaluation of four different strategies to characterize plasma membrane proteins from banana roots]Evaluation of four different strategies to characterize plasma membrane proteins from banana roots
Keller, E.R.J.Panis, B.Engelmann, F.[In vitro storage and cryopreservation as substantial complements in concerted actions to better maintain and use crop germplasm]In vitro storage and cryopreservation as substantial complements in concerted actions to better maintain and use crop germplasm

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