This publication describes cryopreservation methods developed for Musa tissue at KUL (Katholieke Universiteit Leuven, Belgium) and JIRCAS (Japanese International Research Centre for Agricultural Sciences). Detailed protocols are given for all steps to follow from the preparation of the plant mate...
Panis, B.
,
Thinh, N.T.
,
International Network for the Improvement of Banana and Plantain
,
[Cryopreservation of Musa germplasm]
,
Cryopreservation of Musa germplasm
This guideline presents two protocols to produce embryogenic cell suspensions by using scalps or immature male flowers. The protocols have been developed by the Laboratory of Tropical Crop Improvement of Katholieke Universiteit Leuven (KULeuven) and the cellular biology laboratory of the Centre d...
Srosse, H.
,
Domergue, R.
,
Panis, B.
,
Escalant, J-V.
,
Côte, F.
,
Vézina, A.
,
Picq, C.
,
International Network for the Improvement of Banana and Plantain
,
[Suspensiones de células embriogénicas de banano y plátano]
,
Suspensiones de células embriogénicas de banano y plátano
This guideline presents two protocols to produce embryogenic cell suspensions by using scalps or immature male flowers. The protocols have been developed by the Laboratory of Tropical Crop Improvement of Katholieke Universiteit Leuven (KULeuven) and the cellular biology laboratory of the Centre d...
Srosse, H.
,
Domergue, R.
,
Panis, B.
,
Escalant, J-V.
,
Côte, F.
,
Vézina, A.
,
Picq, C.
,
International Network for the Improvement of Banana and Plantain
,
[Suspensions cellulaires embryogènes de bananiers et bananiers plantain]
,
Suspensions cellulaires embryogènes de bananiers et bananiers plantain
Banana and plantain (Musa spp.) are the fourth largest food commodity after rice, wheat, and milk in terms of the gross value of production (CGIAR 1992). They are produced in the humid tropics of many developing economies; about 74 million tons are produced annually (FAO 1991).
We have developed a simple protocol to allow the production of transgenic banana plants. Foreign genes were delivered into embryogenic suspension cells using accelerated particles coated with DNA. Bombardment parameters were optimized for a modified particle gun resulting in high levels of transi...
Sági, L.
,
Panis, B.
,
Remy, S.
,
Schoofs, H.
,
Smet, K. de
,
Swennen, R.
,
Cammue, B.P.
,
[Genetic transformation of banana and plantain (Musa spp.) via particle bombardment]
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Genetic transformation of banana and plantain (Musa spp.) via particle bombardment