Radopholus similisis a major pest of East African highland cooking bananas (Musaspp.) in Uganda. Non-pathogenic Fusariumoxysporumendophytes, isolated from bananas in farmers’ fields, have shown potential to reduceR. Similis numbers in tissue culture banana. The mechanism through which endophyte...
Paparu, P.
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Dubois, T.
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Coyne, D.L.
,
Viljoen, A.
,
[Defenserelated gene expression in susceptible and tolerant bananas (Musa spp.) following inoculation with nonpathogenic Fusarium oxysporum endophytes and challenge with Radopholus similis]
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Defenserelated gene expression in susceptible and tolerant bananas (Musa spp.) following inoculation with nonpathogenic Fusarium oxysporum endophytes and challenge with Radopholus similis
Beauveria bassiana is considered a virulent pathogen against the banana weevil Cosmopolites sordidus. However, current field application techniques for effective control against this pest remain a limitation and an alternative method for effective field application needs to be investigated. Three...
Akello, J.
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Dubois, T.
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Gold, C.S.
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Coyne, D.L.
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Nakavuma, J.
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Paparu, P.
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[Beauveria bassiana (balsamo) vuillemin as an endophyte in tissue 3 culture banana (Musa spp.)]
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Beauveria bassiana (balsamo) vuillemin as an endophyte in tissue 3 culture banana (Musa spp.)
The use of mutualistic fungal endophytes to control the banana weevil (Cosmopolites sordidus) and banana parasitic nematodes (Radopholus similis, Pratylenchus goodeyi and Helicotylenchus multicinctus) is currently being investigated at the International Institute of Tropical Agriculture. Fungal e...
Paparu, P.
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Dubois, T.
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Gold, C.S.
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Adipala, E.
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Niere, B.
,
Coyne, D.
,
[Inoculation, colonization, and distribution of fungal endophytes in Musa tissue culture plants]
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Inoculation, colonization, and distribution of fungal endophytes in Musa tissue culture plants
Among the major constriats to highland cooking banana (Musa spp. AAA-EA) production in Uganda is the high pests infestation levels. Most important are the banana weevil cosmopolites sordidus and a complex of plant parasitic root nematodes of which Radopholus similis is the most important. Clean p...
Paparu, P.
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Dubois, T.
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Athman, S.
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Kapindu, S.
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Coyne, D.
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Gold, C.
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Adipala, E.
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Mukwaba, E.
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[Merging biotechnology with biological control: banana Musa tissue culture plants enhanced by endophytic fungi]
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Merging biotechnology with biological control: banana Musa tissue culture plants enhanced by endophytic fungi
Non-pathogenic endophytic Fusarium oxysporum inoculated into banana (Musa spp.) tissue culture plants can provide protection against banana weevils (Cosmopolites sordidus) and nematodes (Radopholus similis). The degree of control probably depends, in part, upon the level of endophyte establishmen...
Paparu, P.
,
Dubois, T.
,
Gold, C.
,
Niere, B.
,
Adipala, E.
,
Coyne, D.
,
[Improved colonization of East African highland Musa tissue culture plants by endophytic Fusarium oxysporum]
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Improved colonization of East African highland Musa tissue culture plants by endophytic Fusarium oxysporum
The burrowing nematode (Radopholus similis (Cobb) Thorne) and the banana weevil (Cosmopolites sordidus Germar, Coleoptera: Curculionidae) are major pests of banana (Musa spp.) in the Lake Victoria basin region of Uganda. Among biological options to control the two pests is the use of non-pathogen...
Paparu, P.
,
Dubois, T.
,
Coyne, D.L.
,
Viljoen, A.
,
[Dual inoculation of Fusarium oxysporum endophytes in banana: effect on plant colonization, growth and control of the root burrowing nematode and the banana weevil]
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Dual inoculation of Fusarium oxysporum endophytes in banana: effect on plant colonization, growth and control of the root burrowing nematode and the banana weevil
In studying plant colonization by inoculated Fusarium oxysporum endophytes, it is important to be able to distinguish inoculated isolates from saprophytic strains. In the current study, F. oxysporum isolates were transformed with the green (GFP) and red fluorescent protein (DsRed) genes, and beno...
Paparu, P.
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Macleod, A.
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Dubois, T.
,
Coyne, D.L.
,
Viljoen, A.
,
[Efficacy of chemical and flourescent protein markers in studying plant colonization by endophytic nonpathogenic Fusarium oxysporum isolates]
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Efficacy of chemical and flourescent protein markers in studying plant colonization by endophytic nonpathogenic Fusarium oxysporum isolates
The root burrowing nematode Radopholus similis and the banana weevil Cosmopolites sordidus are major pests of banana in the Lake Victoria basin region. An integrated pest management approach that includes habitat management, biological control, host plant resistance and chemical control is curren...
Paparu, P.
,
Dubois, T.
,
Coyne, D.L.
,
Viljoen, A.
,
[Effect of dual endophyte inoculations on plant colonization, and control of Radopholus similis and the banana weevil]
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Effect of dual endophyte inoculations on plant colonization, and control of Radopholus similis and the banana weevil
Under laboratory conditions, nonpathogenic, endophytic Fusarium oxysporum inflicts high mortality among banana weevils and nematodes. Following inoculation into banana (Musa spp.) tissue cultured plants, successful colonisation is necessary for efficient biological control of these pests. The pat...
Paparu, P.
,
Dubois, T.
,
Gold, C.
,
Niere, B.
,
Adipala, E.
,
Coyne, D.L.
,
[Colonisation pattern of nonpathogenic Fusarium oxysporum, a potential biological control agent, in roots and rhizomes of tissue cultured Musa plantlets]
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Colonisation pattern of nonpathogenic Fusarium oxysporum, a potential biological control agent, in roots and rhizomes of tissue cultured Musa plantlets