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Pelle, Roger[The use of electrophoretic profile of RNA to differentiate Trypanosoma brucei from T. congolense]The use of electrophoretic profile of RNA to differentiate Trypanosoma brucei from T. congolense

East Coast fever (ECF) is a highly fatal lymphoproliferative disease of cattle caused by Theileria parva, a tick-borne intracellular apicomplexan parasite. Parasite antigens that are targets of protective cytotoxic T lymphocyte (CTL) responses are required to formulate a sub-unit vaccine against ...


Akoolo, L.Pelle, RogerSaya, R.Awino, EliasNyanjui, J.Taracha, E.L.N.Kanyari, P.Mwangi, D.M.Graham, S.P.[Evaluation of the recognition of Theileria parva vaccine candidate antigens by cytotoxic T lymphocytes from Zebu cattle]Evaluation of the recognition of Theileria parva vaccine candidate antigens by cytotoxic T lymphocytes from Zebu cattle

Immunity against the bovine intracellular protozoan parasite Theileria parva has been shown to be mediated through the destruction of parasite infected lymphocytes by MHC class I restricted CD8+ CTL. Six parasite proteins targeted by CTL from T. parva immune cattle have been identified and raised...


Graham, S.P.Pelle, RogerVilliers, Etienne P. deYamage, M.Mwangi, D.M.Honda, Y.Ellis, S.A.MacHugh, Niall D.Morrison, W. IvanTaracha, E.L.N.[Identification of bovine cytotoxic T lymphocyte epitopes on the intracellular parasite Theileria Parva]Identification of bovine cytotoxic T lymphocyte epitopes on the intracellular parasite Theileria Parva

Theileria parva antigens recognized by cytotoxic T lymphocytes (CTLs) are prime vaccine candidates against East Coast Fever in cattle. A strategy for enhancing induction of parasite-specific T cell responses by increasing recruitment and activation of dendritic cells (DCs) at the immunization sit...


Mwangi, D.M.Honda, Y.Graham, S.P.Pelle, RogerTaracha, E.L.N.Gachanja, J.Nyanjui, J.K.Bray, J.Palmer, G.H.Brown, W.C.Mwangi, W.[Treatment of cattle with DNA-encoded Flt3L and GM-CSF prior to immunization with Theileria parva candidate vaccine antigens induces CD4 and CD8 T cell IFN-γ responses but not CTL responses]Treatment of cattle with DNA-encoded Flt3L and GM-CSF prior to immunization with Theileria parva candidate vaccine antigens induces CD4 and CD8 T cell IFN-γ responses but not CTL responses

N-Ribohydrolases, including the inosine-adenosine-guanosine-preferring (IAG) nucleoside hydrolase, have been proposed to be involved in the nucleoside salvage pathway of protozoan parasites and may consitute rational therapeutic targets for the treatment of these diseases. Reported is the complet...


Pelle, RogerSchramm, V.L.Parkin, D.W.[Molecular cloning and expression of a purine-specific N-ribohydrolase from Trypanosoma brucei brucei. Sequence expression and molecular analysis]Molecular cloning and expression of a purine-specific N-ribohydrolase from Trypanosoma brucei brucei. Sequence expression and molecular analysis

Recent studies suggest that peptidyl-prolyl isomerases of the cyclophilin family, that access the secretory pathway, can be involved in the interaction of parasitic protozoa with mammalian host cells. The amino acid sequence of a cDNA encoding a cyclophilin family member of the intracellular pro...


Ebel, T.Pelle, RogerJanoo, R.T.K.Lipp, J.Bishop, Richard P.[A membrane-anchored Theileria parva cyclophilin with a non-cleaved amino-terminal signal peptide for entry into the endoplasmic reticulum]A membrane-anchored Theileria parva cyclophilin with a non-cleaved amino-terminal signal peptide for entry into the endoplasmic reticulum
Pelle, RogerMurphy, N.B.[In vivo UV cross-linking hybridization, a powerful technique for isolating RNA binding proteins: Application to trypanosome mini-exon derived RNA]In vivo UV cross-linking hybridization, a powerful technique for isolating RNA binding proteins: Application to trypanosome mini-exon derived RNA

The purine salvage pathway of parasitic protozoa is currently considered as a target for drug development because these organisms cannot synthesize purines de novo. Insight into the structure and mechanism of the involved enzymes can aid in the development of potent inhibitors, leading to new cur...


Versees, W.Decanniere, K.Pelle, RogerDepoorter, J.Brosens, E.Parkin, D.W.Steyaert, J.[Structure and function of a novel purine specific nucleoside hydrolase from Trypanosoma vivax]Structure and function of a novel purine specific nucleoside hydrolase from Trypanosoma vivax

Cyclophilin A from the bovine parasite Trypanosoma brucei brucei has been cloned, expressed in Escherichia coli, purified and crystallized in the presence of cyclosporin A using ammonium sulfate as a precipitant. The crystals belong to the orthorhombic crystal system with unit-cell dimensions of ...


Dao-Thi, M.H.Transue, T.R.Pelle, RogerMurphy, N.B.Poortmans, F.Steyaert, J.[Expression, purification, crystalization and preliminary X-ray analysis of cyclophilin A from the bovine parasite Trypanosoma brucei brucei]Expression, purification, crystalization and preliminary X-ray analysis of cyclophilin A from the bovine parasite Trypanosoma brucei brucei

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