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Gray, M.A.Kimarua, R.W.Peregrine, A.S.[Drug sensitivity screening in vitro of populations of Trypanosoma congolense originating from cattle and tsetse flies at Nguruman, Kenya]Drug sensitivity screening in vitro of populations of Trypanosoma congolense originating from cattle and tsetse flies at Nguruman, Kenya

A method is described for calculating new infection incidence from monthly field data collected between April 1994 and February 1998 from an average of 770 Ethiopian Highland Zebu cattle maintained under traditional management in the Ghibe valley, southwest Ethiopia and exposed to a high challeng...


Rowlands, G.J.Leak, S.G.A.Peregrine, A.S.Nagda, S.M.Mulatu, W.D'Ieteren, G.D.M.[The incidence of new and the prevalence and persistence of recurrent trypanosome infections in cattle in southewest Ethiopia exposed to a high challenge with drug-resistant parasites]The incidence of new and the prevalence and persistence of recurrent trypanosome infections in cattle in southewest Ethiopia exposed to a high challenge with drug-resistant parasites

Ten trypanosome isolates were collected at random from cattle at Ghibe, Ethiopia, in February 1993 and all shown to be savannah-type Trypanosoma congolense. When inoculated into naive Boran (Bos indicus) calves, all 10 isolates were resistant to diminazene aceturate (Berenil), isometamidium chlor...


Mulugeta, W.Wilkes, J.Mulatu, W.Majiwa, P.A.O.Masake, R.Peregrine, A.S.[Long-term occurrence of Trypanosoma congolense resistant to diminazene, isometamidium and homidium in cattle at Ghibe, Ethiopia]Long-term occurrence of Trypanosoma congolense resistant to diminazene, isometamidium and homidium in cattle at Ghibe, Ethiopia

Testing of trypanocidal drug sensitivity in stabilates of pathogenic trypanosomes derived from cattle continues to be problematic, in spite of Development of in vitro techniques, since these are generally complicated and costly for the resources available in developing country laboratories. Furth...


Geerts, S.Ndung'u, J.M.Murilla, G.Mbwambo, H.Sinyangwe, L.Machila, N.Delespaux, V.Brandt, J.Peregrine, A.S.McDermott, John J.Holmes, P.H.Eisler, M.C.[A simplified cost-effective method for area-wide testing of trypanocidal drug sensitivity of T. congolense in mice]A simplified cost-effective method for area-wide testing of trypanocidal drug sensitivity of T. congolense in mice

In a cross-sectional study carried out in June 1994, 486 cattle from 50 randomly selected dairy farms in the vicinity of Kampala, Uganda, were examined for trypanosome infections. The trypanosome prevalence (either positive with the haematocrit centrifugation technique [HCT] and/or the mini-anion...


Clausen, P.H.Schares, G.Patzelt, R.J.Poetzsch, C.J.Scheer, A.Kakaire, D.Olila, D.Peregrine, A.S.McDermott, John J.Mehlitz, D.[PCR and DNA-probe hybridization to assess the efficacy of therapeutic and prophylactic isometamidium treatment in Trypanosoma spp. - infected dairy cattle in peri-urban Kampala, Uganda]PCR and DNA-probe hybridization to assess the efficacy of therapeutic and prophylactic isometamidium treatment in Trypanosoma spp. - infected dairy cattle in peri-urban Kampala, Uganda

Control of trypanosomes in cattle, sheep and goats in endemic areas has depended largely on the use of chemotherapeutic or chemoprophylactic agents. One such agent is homidium. The normal use of homidium has been in the treatment of infections due to both Trypanosoma congolense and T.vivax in cat...


Murilla, G.A.Peregrine, A.S.Holmes, P.H.Eisler, M.C.Ndung'u, J.M.[Investigation into the effects of Trypanosoma congolense infections on the pharmacokinetics of Homidium in Boran cattle]Investigation into the effects of Trypanosoma congolense infections on the pharmacokinetics of Homidium in Boran cattle

Resistance to the drugs used to control African animal trypanosomosis is increasingly recognised as a constraint to livestock production in sub-Saharan Africa. The most commonly used tests for detection of trypanocidal drug resistance are tests using mice or ruminants, but these suffer from lack ...


Eisler, M.C.Brandt, J.Bauer, B.Clausen, P.H.Delespaux, V.Holmes, P.H.Ilemobade, A.Machila, N.Mbwambo, H.McDermott, John J.Mehlitz, D.Murilla, G.Ndung'u, J.M.Peregrine, A.S.Sidibe, I.Sinyangwe, L.Geerts, S.[Standardised tests in mice and cattle for the detection of drug resistance in tsetse-transmitted trypanosomes of African domestic cattle]Standardised tests in mice and cattle for the detection of drug resistance in tsetse-transmitted trypanosomes of African domestic cattle
Waitumbi, J.N.Murphy, N.B.Peregrine, A.S.[Genotype and drug-resistance phenotype of Trypanosoma evansi originating from camels in Northern Kenya]Genotype and drug-resistance phenotype of Trypanosoma evansi originating from camels in Northern Kenya
Peregrine, A.S.Ogunyemi, O.Whitelaw, D.D.Holmes, P.H.Moloo, S.K.Hirumi, H.Urquhart, G.M.Murray, M.[Factors influencing the duration of isometamidium chloride (Samorin) prophylaxis against experimental challenge with metacyclic forms of Trypanosoma congolense]Factors influencing the duration of isometamidium chloride (Samorin) prophylaxis against experimental challenge with metacyclic forms of Trypanosoma congolense

Bloodstream forms of four populations of the livestock pathogen Trypanosoma congolense, isolated from different natural infections, have been shown to exhibit a wide range of sensitivities to the trypanocide isometamidium chloride (Samorin). In mice the 50 percent curative doses (CD 50) for Samor...


Wilkes, J.M.Mulugeta, W.Wells, C.Peregrine, A.S.[Modulation of mitochondrial electrical potential: A candidate mechanism for drug resistance in African trypanosomes]Modulation of mitochondrial electrical potential: A candidate mechanism for drug resistance in African trypanosomes

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