Resistance to isometamidium was increased 94-fold in a clone of Trypanosoma congolense (clone IL 1180) by repeated subcurative treatment of infected mice for 11 months. This was associated with 3.4-, 33-, and 4.2-fold increases in resistance to diminazene, homidium, and quinapyramine, respectivel...
Peregrine, A.S.
,
Gray, M.A.
,
Moloo, S.K.
,
[Cross-resistance associated with development resistance to isometamidium in a clone of Trypanosoma congolense]
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Cross-resistance associated with development resistance to isometamidium in a clone of Trypanosoma congolense
Murphy, N.B.
,
Muthiani, M.
,
Peregrine, A.S.
,
[Use of an in vivo system to determine the G418 resistance phenotype of bloodstream-form Trypanosoma brucei brucei transfectants]
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Use of an in vivo system to determine the G418 resistance phenotype of bloodstream-form Trypanosoma brucei brucei transfectants
Interaction of the trypanocide isometamidium chloride with components of Trypanosoma congolense results in characteristic shifts in the intrinsic fluorescence of the drug. The specificity of this interaction was investigated by analysing the effects of various physiochemical manipulations on its ...
Wilkes, J.M.
,
Peregrine, A.S.
,
Zilberstein, D.
,
[The accumulation and compartmentalization of isometamidium chloride in Trypanosoma congolense, monitored by its intrinsic fluorescence]
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The accumulation and compartmentalization of isometamidium chloride in Trypanosoma congolense, monitored by its intrinsic fluorescence
Ndoutamia, G.
,
Moloo, S.K.
,
Murphy, N.B.
,
Peregrine, A.S.
,
[Derivation and characterization of a quinapyramine-resistant clone of Trypanosoma congolense]
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Derivation and characterization of a quinapyramine-resistant clone of Trypanosoma congolense
Sutherland, I.A.
,
Codjia, V.
,
Moloo, S.K.
,
Holmes, P.H.
,
Peregrine, A.S.
,
[Therapeutic activity of isometamidium chloride in Boran cattle against a clone of Trypanosoma congolense with a low level of drug resistance]
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Therapeutic activity of isometamidium chloride in Boran cattle against a clone of Trypanosoma congolense with a low level of drug resistance
Two groups of five Boran (Bos indicus) cattle were infected with one of two populations of Trypanosoma congolense; one drug-sensitive (IL1180), and one drug-resistant (IL3330). The animals were then treated intramuscularly with homidium bromide at a dose rate of 1.0 mg kg - bodyweight 7 days afte...
Murilla, G.A.
,
Peregrine, A.S.
,
Ndung'u, J.M.
,
Holmes, P.H.
,
Eisler, M.C.
,
[The effects of drug-sensitive and drug-resistant Trypanosoma congolense infections on the pharmacokinetics of homidium in Boran cattle]
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The effects of drug-sensitive and drug-resistant Trypanosoma congolense infections on the pharmacokinetics of homidium in Boran cattle
Two enzyme-linked immunosorbent assays (ELISA) for the determination of homidium in serum of treated cattle have been developed and evaluated. One is a direct competition (Assay 1) and the other an indirect competition assay (Assay 2). Both assays are highly sensitive with a limit of detection of...
Murilla, G.
,
Eisler, M.C.
,
Peregrine, A.S.
,
Ndung'u, J.M.
,
Holmes, P.H.
,
[Development and evaluation of an enzyme-linked immunosorbent assay (ELISA) for the determination of the trypanocidal drug homidium in serum of treated cattle]
,
Development and evaluation of an enzyme-linked immunosorbent assay (ELISA) for the determination of the trypanocidal drug homidium in serum of treated cattle
Pharmacokinetic studies on the trypanocidal drug homidium bromide using a competitive enzyme immunoassay (detection limit 0.1 ng/mL) are reported for non-infected Friesian and Boran steers following treatment with homidium bromide at a dose of 1.0 mg/kg b.w. Following intravenous (i.v.) treatment...
Murilla, G.
,
Holmes, P.H.
,
Peregrine, A.S.
,
Eisler, M.C.
,
Ndung'u, J.M.
,
[Some pharmacokinetic parameters of the trypanocidal drug homidium bromide in Friesian and Boran steers using an enzyme-linked immunosorbent assay (ELISA)]
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Some pharmacokinetic parameters of the trypanocidal drug homidium bromide in Friesian and Boran steers using an enzyme-linked immunosorbent assay (ELISA)
Control of trypanosomosis in cattle, sheep and goats in endemic areas has depended largely on the use of chemotherapeutic or chemoprophylactic agents. One such agent is homidium. The normal use of homidium has been in the treatment of infections due to both Trypanosoma congolense and T. vivax in ...
Murilla, G.
,
Peregrine, A.S.
,
Holmes, P.H.
,
Eisler, M.C.
,
Ndung'u, J.M.
,
[Investigation into the effects of Trypanosoma congolense infections on the pharmacokinetics of Homidium in Boran cattle]
,
Investigation into the effects of Trypanosoma congolense infections on the pharmacokinetics of Homidium in Boran cattle