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Murphy, N.B.Muthiani, C.Pandit, P.Kemei, S.Moloo, S.K.Hirumi, K.Hirumi, H.Peregrine, A.S.[The identification of genes of importance in Trypanosoma brucei brucei through the use of transfection techniques]The identification of genes of importance in Trypanosoma brucei brucei through the use of transfection techniques

Peregrine, A.S.Sutherland, I.A.Mutharia, L.Jamnadass, Ramni H.Gray, M.A.Codjia, V.Aliu, Y.O.Mamman, M.Silayo, R.D'Ieteren, G.D.M.Moloo, S.K.Murphy, N.B.[Overview of the chemotherapy research program at ILRAD]Overview of the chemotherapy research program at ILRAD

Testing of trypanocidal drug sensitivity in stabilates of pathogenic trypanosomes derived from cattle continues to be problematic, in spite of Development of in vitro techniques, since these are generally complicated and costly for the resources available in developing country laboratories. Furth...


Geerts, S.Ndung'u, J.M.Murilla, G.Mbwambo, H.Sinyangwe, L.Machila, N.Delespaux, V.Brandt, J.Peregrine, A.S.McDermott, John J.Holmes, P.H.Eisler, M.C.[A simplified cost-effective method for area-wide testing of trypanocidal drug sensitivity of T. congolense in mice]A simplified cost-effective method for area-wide testing of trypanocidal drug sensitivity of T. congolense in mice

In a cross-sectional study carried out in June 1994, 486 cattle from 50 randomly selected dairy farms in the vicinity of Kampala, Uganda, were examined for trypanosome infections. The trypanosome prevalence (either positive with the haematocrit centrifugation technique [HCT] and/or the mini-anion...


Clausen, P.H.Schares, G.Patzelt, R.J.Poetzsch, C.J.Scheer, A.Kakaire, D.Olila, D.Peregrine, A.S.McDermott, John J.Mehlitz, D.[PCR and DNA-probe hybridization to assess the efficacy of therapeutic and prophylactic isometamidium treatment in Trypanosoma spp. - infected dairy cattle in peri-urban Kampala, Uganda]PCR and DNA-probe hybridization to assess the efficacy of therapeutic and prophylactic isometamidium treatment in Trypanosoma spp. - infected dairy cattle in peri-urban Kampala, Uganda

Control of trypanosomes in cattle, sheep and goats in endemic areas has depended largely on the use of chemotherapeutic or chemoprophylactic agents. One such agent is homidium. The normal use of homidium has been in the treatment of infections due to both Trypanosoma congolense and T.vivax in cat...


Murilla, G.A.Peregrine, A.S.Holmes, P.H.Eisler, M.C.Ndung'u, J.M.[Investigation into the effects of Trypanosoma congolense infections on the pharmacokinetics of Homidium in Boran cattle]Investigation into the effects of Trypanosoma congolense infections on the pharmacokinetics of Homidium in Boran cattle

Control of trypanosomosis in cattle, sheep and goats in endemic areas has depended largely on the use of chemotherapeutic or chemoprophylactic agents. One such agent is homidium. The normal use of homidium has been in the treatment of infections due to both Trypanosoma congolense and T. vivax in ...


Murilla, G.Peregrine, A.S.Holmes, P.H.Eisler, M.C.Ndung'u, J.M.[Investigation into the effects of Trypanosoma congolense infections on the pharmacokinetics of Homidium in Boran cattle]Investigation into the effects of Trypanosoma congolense infections on the pharmacokinetics of Homidium in Boran cattle

Drug resistance in trypanosomes is increasingly being recognised as a constraint to livestock production in many parts of Africa. However the long-term stability of such resistance is unclear and has therefore been investigated over a 4-year period at a site in Ethiopia. The result shows that the...


Peregrine, A.S.Majiwa, P.A.O.Rowlands, G.J.[Long-term occurrence of multiple drug resistance in Trypanosoma congolense at Ghibe, Ethiopia]Long-term occurrence of multiple drug resistance in Trypanosoma congolense at Ghibe, Ethiopia

Characterising the drug susceptibility of trypanosomes that are pathogenic for domestic livestock has for many years been carried out either in vivo, using large or small animals, or by using various in vitro cultivation methodologies (Peregrine, 1994). Thus, adaptation to, and multiplication of ...


Peregrine, A.S.Wells, C.W.Wilkes, J.M.[Methods for detection of drug resistance in trypanosomes - new developments?]Methods for detection of drug resistance in trypanosomes - new developments?

Trypanosomosis remains one of the biggest impediments to the Development of the livestock industry in sub-Saharan Africa while it is likely that a vaccine for this disease could be found in the future, none is presently available. Mean-while different strategies are being followed to control this...


Majiwa, P.A.O.Konde, V.Souza, M. dePeregrine, A.S.Maina, M.[Molecular approaches to the identification of DNA markers for drug resistance in Trypanosoma congolense]Molecular approaches to the identification of DNA markers for drug resistance in Trypanosoma congolense

Trypanocidal drugs are the most widely applied method of trypanosomosis control in Africa and the only control method in Latin America and Asia . Treatment recommendations and strategies for chemotherapeutic control of trypanosomosis depends on information of trypanosomosis risk and the prevalenc...


Eisler, M.C.McDermott, John J.Mdachi, R.Murilla, G.Sinyangwe, L.Mubanga, J.Machila, N.Mbwambo, H.Coleman, P.G.Clausen, P.H.Bauer, B.Sidibe, I.Geerts, S.Holmes, P.H.Peregrine, A.S.[Rapid method for the assessment of trypanocidal drug resistance in the field]Rapid method for the assessment of trypanocidal drug resistance in the field

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