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Seed production from hybridizations involving the East African Highland bananas (Mum spp., AAA group) is low due to high levels of sterility. This is a major impediment for the development of hybrids with disease and pest resistance and superior agronomic traits. The objective of this study was t...


Ssebuliba, R.Magambo, M.Talengera, D.Makumbi, DanTenkouano, A.Rubaihayo, P.R.Pillay, M.[Biological factors affecting seed set in highland bananas]Biological factors affecting seed set in highland bananas

Poor reproductive development in yams (Dioscorea spp.) has often been attributed to the polyploid nature of the crop. In this study, flow cytometry was used to determine the ploidy level of 53 accessions of Dioscorea alata, mostly from West African countries, Chad and Puerto Rico. Nuclei were iso...


Egesi, Chiedozie N.Pillay, M.Asiedu, A.Egunjobi, J.K.[Ploidy analysis in water yam, Dioscorea alata L., germplasm]Ploidy analysis in water yam, Dioscorea alata L., germplasm
Blomme, GuyPrice, N.Coyne, D.L.Lepoint, P.Nicolas, N.Ndayihazamaso, P.Niyongere, C.Yemataw, Z.Addis, T.Pillay, M.Karamura, E.B.Jones, D.[Current geographical distribution of Xanthomonas wilt of banana and enset, and some nematode species]Current geographical distribution of Xanthomonas wilt of banana and enset, and some nematode species

The ploidy levels of the twenty-two yam (Dioscorea cayenensis-D. rotundata complex) cultivars within germplasm Cameroon Guinea yam were determined by flow cytometry. Three different ploidy levels (4x,6x, 8x) were detected within the samples analysed. Fifteen cultivars were tetraploids, five were ...


Dansi, A.Mignouna, H.Pillay, M.Zok, S.[Ploidy variation in the cultivated yams (Dioscorea cayenensisDioscorea rotundata complex) from Cameroon as determined by flow cytometry]Ploidy variation in the cultivated yams (Dioscorea cayenensisDioscorea rotundata complex) from Cameroon as determined by flow cytometry

Plantains and bananas (Musa spp. sect. eumusa) originated from intra- and interspecific hybridization between two wild diploid species, M. acuminata Colla. and M. balbisiana Colla., which contributed the A and B genomes, respectively. Polyploidy and hybridization have given rise to a number of di...


Pillay, M.Nwakanma, D.Tenkouano, A.[Identification of RAPD markers linked to A and B genome sequences in Musa L]Identification of RAPD markers linked to A and B genome sequences in Musa L

The ploidy level of ninety Guinea yam (Dioscorea Cayenesis/D. rotundata complex) cultivars identified within Benin were determined by both chromosome counting from root tip cells and flow cytometry. Three different ploidy levels (4x,6x,8x,) were detected among the samples. Eighty cultivars were t...


Dansi, A.Pillay, M.Mignouna, H.Daïnou, O.Mondeil, F.Moutairou, K.[Ploidy level of the cultivated yams (Dioscorea cayenensis/D. rotundata complex) from Benin Republic as determined by chromosome counting and flow cytometry]Ploidy level of the cultivated yams (Dioscorea cayenensis/D. rotundata complex) from Benin Republic as determined by chromosome counting and flow cytometry

Musa spp (bananas and plantains) constitute a hybrid-polyploid complex and are classified according to different genome compositions such as AA, BB, AB, AAA, AAB, ABB, AAAA, ABBB, AAAB and AABB. Knowledge of ploidy and exact genome compositions of the parental material is essential for Musa breed...


Pillay, M.Ogundiwin, E.Tenkouano, A.Doležel, Jaroslav[Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA)]Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA)

Musa acuminata Colla (AA genomes) and Musa balbisiana Colla (BB genomes) are the diploid ancestors of modern bananas that are mostly diploid or triploid cultivars with various combinations of the A and B genomes, including AA, AAA, BB, AAB and ABB. The objective of this study was to identify mole...


Nwakanma, D.C.Pillay, M.Okoli, B.E.Tenkouano, A.[PCRRFLP of the ribosomal DNA internal transcribed spacers (ITS) provides markers for the A and B genomes in Musa L.]PCRRFLP of the ribosomal DNA internal transcribed spacers (ITS) provides markers for the A and B genomes in Musa L.

The objective of this study was to construct a molecular phylogeny of the genus Musa using restriction-site polymorphisms of the chloroplast (cpDNA) and mitochondrial DNA (mtDNA). Six cpDNA and two mtDNA sequences were amplified individually in polymerase chain reaction (PCR) experiments in 13 sp...


Nwakanma, D.C.Pillay, M.Okoli, B.E.Tenkouano, A.[Sectional relationships in the genus Musa L. inferred from PCRRFLP of organelle DNA sequences]Sectional relationships in the genus Musa L. inferred from PCRRFLP of organelle DNA sequences

Fifteen AFLP primer pairs (EcoRI+3 and MseI+3) and 60 10-mer RAPD primers were used to detect polymorphisms and assess genetic relationships in a sample of 25 plantains from diverse parts of Western and Central Africa. The discriminatory power of the AFLP technique was greater than that of the RA...


Ude, G.Pillay, M.Ogundiwin, E.Tenkouano, A.[Genetic diversity in an African plantain core collection using AFLP and RAPD markers]Genetic diversity in an African plantain core collection using AFLP and RAPD markers

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