Meiotic studies in Musa L. have been hampered by: 1) time-consuming efforts required to find the correct stages of cell division; 2) rigidity of the microsporocyte cell wall that makes preparation of smears difficult; and 3) poor staining of prophase chromosomes. This study describes an improved ...
Adeleke, M.
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Pillay, M.
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Okoli, B.E.
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[An improved method for examining meiotic chromosomes in Musa L.]
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An improved method for examining meiotic chromosomes in Musa L.
Genetic diversity and relationships were assessed in 28 accessions of Musa acuminata (AA) Colla and Musa balbisiana (BB) Colla, and some of their natural hybrids, using the amplified fragment length poly-morphisms (AFLP) technique. Fifteen AFLP +3 primer pairs produced 527 polymorphic bands among...
Ude, G.
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Pillay, M.
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Nwakanma, D.
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Tenkouano, A.
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[Genetic diversity in Musa acuminata Colla and Musa balbisiana Colla and some of their natural hybrids using AFLP markers]
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Genetic diversity in Musa acuminata Colla and Musa balbisiana Colla and some of their natural hybrids using AFLP markers
Banana is an important food and cash crop in Uganda. The crop displays wide diversity but the genetic relationship between and withing cultivars is largely unknown. A study was conducted to assess genetic relationships among 'Sukali Ndizi' clones collected from 16 different localities in Uganda ...
Pillay, M.
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Dimkpa, C.O.
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Ude, G.
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Makumbi, Dan
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Tushemereirwe, W.K.
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[Genetic variation in the banana cultivar Sukali ndizi grown in different regions of Uganda]
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Genetic variation in the banana cultivar Sukali ndizi grown in different regions of Uganda
The majority of edible bananas (Musa L.) are triploid (2n = 3x = 33) cultivars known as dessert, plantain, or cooking bananas with predominant AAA, AAB, and ABB genomes, justifying the aim of breeding programs to develop improved hybrids of the different utilization classes in the triploid backgr...
Oselebe, H.O.
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Tenkouano, A.
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Pillay, M.
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Obi, I.U.
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Uguru, M.I.
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[Ploidy and genome segregation in Musa breeding populations assessed by flow cytometry and randomly amplified polymorphic DNA markers]
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Ploidy and genome segregation in Musa breeding populations assessed by flow cytometry and randomly amplified polymorphic DNA markers
The improvement of East African highland bananas (EAHBs) using conventional breeding methods is difficult due to their biology and therefore focus on improving them has shifted to exploring methods for establishment of embryogenic cell suspensions, which can then be targeted, for genetic transfor...
Sadik, K.
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Rubaihayo, P.R.
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Magambo, M.
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Pillay, M.
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[Generation of cell suspensions of East African highland bananas through scalps]
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Generation of cell suspensions of East African highland bananas through scalps
A quick and reliable method for evaluating pollen quality is essential in a breeding program, especially in a crop such as banana that is characterized by high male and female sterility. In this study the germination and viability of banana pollen was evaluated in a sucrose solution and diluted b...
Nyine, M.
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Pillay, M.
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[Banana nectar as a medium for testing pollen viability and germination in Musa]
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Banana nectar as a medium for testing pollen viability and germination in Musa
Africa is one of the secondary centers of diversity for the genus Musa. There is a wide range of genetic diversity in the bananas found in the continent with different types dominating different ecological sub-regions. Banana production in Africa is affected by a number of diseases and pests. The...
Pillay, M.
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Ssebuliba, R.
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Hartman, J.
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Vuylsteke, D.R.
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Talengera, D.
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Tushemereirwe, W.K.
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[Conventional breeding strategies to enhance the sustainability of Musa biodiversity conservation for endemic cultivars on farm]
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Conventional breeding strategies to enhance the sustainability of Musa biodiversity conservation for endemic cultivars on farm
Cultivated bananas originated from interspecific hybridisation of two wild diploid (2n = 2x = 22) species, Musa acuminata and M. balbisiana that were the donors of the A and B genomes, respectively. Most cultivated bananas are triploids with 2n = 3x = 33 chromosomes. They vary in genome compositi...
Musa spp (bananas and plantains) constitute a hybrid-polyploid complex and are classified according to different genome compositions such as AA, BB, AB, AAA, AAB, ABB, AAAA, ABBB, AAAB and AABB. Knowledge of ploidy and exact genome compositions of the parental material is essential for Musa breed...
Pillay, M.
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Ogundiwin, E.
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Tenkouano, A.
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Doležel, Jaroslav
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[Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA)]
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Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA)