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Rao, Idupulapati M.Wenzl, PeterArango Vélez, AdrianaMiles, John W.Watanabe, ToshihiroShinano, TakuroOsaki, MitsuruWagatsuma, TadaoManrique, GermánBeebe, Stephen E.Tohme, Joseph M.Ishitani, ManabuRangel Becerra, Andrés FelipeHorst, Walter J.[Advances in developing screening methods and improving aluminum resistance in common bean and brachiaria]Advances in developing screening methods and improving aluminum resistance in common bean and brachiaria
Rao, Idupulapati M.Miles, John W.García, RamiroRicaurte Oyola, José Jaumer[Selección de híbridos de Brachiaria con resistencia al aluminnio]Selección de híbridos de Brachiaria con resistencia al aluminnio

We previously developed a new simple technique of methylene blue (MB) staining for the discrimination of aluminum (Al)-tolerant protoplasts from 4 plant species (rice, oats, maize and pea). The objectives of the present study were to confirm the applicability of this technique to a wider range of...


Wagatsuma, TadaoKhan, Shahadat HossainRao, Idupulapati M.Wenzl, PeterTawaraya, KeitarouYamamoto, TakanoriKawamura, TakeshiHosogoe, KazumiIshikawa, Satoru[Methylene blue sustainability of root-tip protoplasts as an indicator of aluminum tolerance in a wide range of plant species, cultivars and lines]Methylene blue sustainability of root-tip protoplasts as an indicator of aluminum tolerance in a wide range of plant species, cultivars and lines

Background and Aims: Aluminium (Al) resistance in common bean is known to be due to exudation of citrate from the root after a lag phase, indicating the induction of gene transcription and protein synthesis. The aims of this study were to identify Al-induced differentially expressed genes and to ...


Eticha, DZahn, MBremer, MYang, ZRangel Becerra, AFRao, Idupulapati M.Horst, Walter J.[Transcriptomic analysis reveals differential gene expression in response to aluminium in common bean (Phaseolus vulgaris) genotypes]Transcriptomic analysis reveals differential gene expression in response to aluminium in common bean (Phaseolus vulgaris) genotypes

Al resistance of signalgrass (Brachiaria decumbens Stapf cv Basilisk), a widely sown tropical forage grass, is outstanding compared with the closely related ruzigrass (Brachiaria ruziziensis Germain and Evrard cv Common) and Al-resistant genotypes of graminaceous crops such as wheat, triticale, a...


Wenzl, PeterPatino, GMChavez, Alba L.Mayer, Jorge EdgardRao, Idupulapati M.[The high level of aluminum resistance in signalgrass is not associated with known mechanism of external aluminum detoxification in root apices]The high level of aluminum resistance in signalgrass is not associated with known mechanism of external aluminum detoxification in root apices

Traditional breeding efforts are expected to be greatly enhanced through collaborative approaches incorporating functional, comparative and structural genomics. Potential benefits of combining genomic tools with traditional breeding have been a source of widespread interest and resulted in numero...


Ishitani, ManabuRao, Idupulapati M.Wenzl, PeterBeebe, Stephen E.Tohme, Joseph M.[Integration of genomics approach with traditional breeding towards improving abiotic stress adaptation: Drought and aluminum toxicity as case studies]Integration of genomics approach with traditional breeding towards improving abiotic stress adaptation: Drought and aluminum toxicity as case studies

The role of the intracellular distribution and binding state of aluminum (Al) in Al toxicity, using Al exchange and Al fractionation methodologies, were studied in two common bean (Phaseolus vulgaris L.) genotypes differing in Al resistance. These two genotypes are characterized by a similar init...


Rangel Becerra, AFRao, Idupulapati M.Horst, Walter J.[Intracellular distribution and binding state of aluminum in root apices of two common bean (Phaseolus vulgaris) genotypes in relation to Al toxicity]Intracellular distribution and binding state of aluminum in root apices of two common bean (Phaseolus vulgaris) genotypes in relation to Al toxicity

Aluminium (Al) toxicity and drought are the two major abiotic stress factors limiting common bean production in the tropics. Using hydroponics, the short-term effects of combined Al toxicity and drought stress on root growth and Al uptake into the root apex were investigated. In the presence of A...


Yang, ZEticha, DRao, Idupulapati M.Horst, Walter J.[Alteration of cell-wall porosity is involved in osmotic stress-induced enhancement of aluminium resistance in common bean (Phaseolus vulgaris L.)]Alteration of cell-wall porosity is involved in osmotic stress-induced enhancement of aluminium resistance in common bean (Phaseolus vulgaris L.)
Manrique Carpintero, GermánRao, Idupulapati M.Beebe, Stephen E.[Identification of aluminum resistant common bean genotypes using a hydroponic screening method]Identification of aluminum resistant common bean genotypes using a hydroponic screening method

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