This study was conducted to determine the use of repeated transvaginal ultrasound-guided cumulus oocyte complex (COC) aspiration on COC recovery rate, in vitro embryo production (IVP) and subsequent pregnancy rates in Holstein Friesian (HF) and Aberdeen Angus (AA) cows (Experiment 1), and in preg...
Ratto, Marcelo
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Peralta, O. A.
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Mogollon, G.
,
Strobel, P.
,
Correa, J.
,
[Transvaginal ultrasound-guided cumulus oocyte complexes aspiration and in vitro embryo production in suckled beef and lactating dairy cattle on pasture-based management conditions]
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Transvaginal ultrasound-guided cumulus oocyte complexes aspiration and in vitro embryo production in suckled beef and lactating dairy cattle on pasture-based management conditions
The objective of the present study was to compare the ovarian follicular response, cumulus-oocyte complex (COL) collection rate, and maturational status of COC collected from alpacas subsequent to treatment with two different superstimulatory protocols. Alpacas (n=7 per group) were treated with: ...
Ratto, Marcelo
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Gomez, Constanza
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Berland Olea, Marco
,
Adams, Gregg P.
,
[Effect of ovarian superstimulation on COC collection and maturation in alpacas]
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Effect of ovarian superstimulation on COC collection and maturation in alpacas
The objective was to determine the effect of energy diet restriction on energy balance, systemic leptin and corpus luteum (CL) vascularization, development, and function in South American camelids. In experiment 1, adult llamas were randomly assigned to receive a diet of 70% of their maintenance ...
Norambuena Ardiles, María
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Hernandez, Francisca
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Alfaro, Jorge
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Silva, Gonzalo
,
Topp, Shirley
,
Ratto, Marcelo
,
[Effect of Different Levels of Energy Diet Restriction on Energy Balance, Leptin and CL Development, Vascularization, and Function in South American Camelids]
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Effect of Different Levels of Energy Diet Restriction on Energy Balance, Leptin and CL Development, Vascularization, and Function in South American Camelids
Gonadotrophin releasing hormone (GnRH) is commonly used in llamas to induce ovulation; however, the consequence of reduced doses of GnRH on luteinizing hormone (LH) release, ovulatory response, and subsequent corpus luteum (CL) development and function have apparently not been investigated. Hence...
Silva Jiménez, Mauricio
,
Colazo, M. G.
,
Ratto, Marcelo
,
[GnRH dose reduction decreases pituitary LH release and ovulatory response but does not affect corpus luteum (CL) development and function in llamas]
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GnRH dose reduction decreases pituitary LH release and ovulatory response but does not affect corpus luteum (CL) development and function in llamas
Norambuena, M. C.
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Silva Jiménez, Mauricio
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Urra, F.
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Ulloa Leal, C.
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Letelier, C.
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Fernandez, A.
,
Adams, G. P.
,
Ratto, Marcelo
,
[EFFECTS OF LONG-TERM NUTRITIONAL RESTRICTION ON PREOVULATORY LUTEINIZING HORMONE SURGE, LEPTIN, AND LUTEAL PHASE IN LLAMAS (LAMA GLAMA)]
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EFFECTS OF LONG-TERM NUTRITIONAL RESTRICTION ON PREOVULATORY LUTEINIZING HORMONE SURGE, LEPTIN, AND LUTEAL PHASE IN LLAMAS (LAMA GLAMA)
The ovulation-inducing effect of seminal plasma was first reported in Bactrian camels over 30 years ago, and the entity responsible was dubbed 'ovulation-inducing factor' (OIF). More recent studies, primarily in llamas and alpacas, characterized the biological and chemical properties of OIF and u...
Adams, G. P.
,
Ratto, Marcelo
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Silva Jiménez, Mauricio
,
Carrasco, R. A.
,
[Ovulation-inducing factor (OIF/NGF) in seminal plasma: a review and update]
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Ovulation-inducing factor (OIF/NGF) in seminal plasma: a review and update
The objectives of the study were to determine the effects of nutritional restriction on ovarian function in llamas. Mature female llamas were assigned randomly to a Control group, fed 100% of maintenance energy requirements (MER) (n = 8), or a Restricted group (n = 8) fed from 70% to 40% of MER u...
Norambuena, M. C.
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Silva Jiménez, Mauricio
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Urra, F.
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Ulloa Leal, C.
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Fernandez, A.
,
Adams, G. P.
,
Huanca, W.
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Ratto, Marcelo
,
[Effects of nutritional restriction on metabolic, endocrine, and ovarian function in llamas (Lama glama)]
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Effects of nutritional restriction on metabolic, endocrine, and ovarian function in llamas (Lama glama)
This study was designed to determine the effect of location of the preovulatory dominant follicle and stage of ovarian follicle development on ovulation rate and embryo survival in alpacas. In Experiment 1, mature lactating alpacas were randomly assigned to one of two groups according to the loca...
Ratto, Marcelo
,
Cervantes, Miriam
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Norambuena Ardiles, María
,
Silva Jiménez, Mauricio
,
Miragaya, Marcelo
,
Huanca, Wilfredo
,
[Effect of location and stage of development of dominant follicle on ovulation and embryo survival rate in alpacas]
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Effect of location and stage of development of dominant follicle on ovulation and embryo survival rate in alpacas
The objectives of the study were to determine the effect of seminal plasma beta-NGF on Corpus Luteum morphology and function and level of mRNA expression of steroidogenic enzymes. Llamas were assigned (n = 12/per group) to receive an intramuscular dose of: (a) 1 ml phosphate buffered saline (PBS)...
Silva Jiménez, Mauricio
,
Ulloa Leal, C.
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Valderrama, X. P.
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Bogle, O. A.
,
Adams, G. P.
,
Ratto, Marcelo
,
[Nerve growth factor from seminal plasma origin (sp beta-NGF) increases CL vascularization and level of mRNA expression of steroidogenic enzymes during the early stage of Corpus Luteum development in llamas]
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Nerve growth factor from seminal plasma origin (sp beta-NGF) increases CL vascularization and level of mRNA expression of steroidogenic enzymes during the early stage of Corpus Luteum development in llamas
This study was designed to: 1) characterize the effect of ovulation-inducing factor (OIF) on pituitary LH secretion in ovariectomized (OVX) llamas; and 2) determine the effect of OIF on LH secretion in OVX llamas pretreated with estradiol-17 beta (E-17 beta) or estradiol benzoate (EB). In Experim...
Silva Jiménez, Mauricio
,
Recabarren, M. P.
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Recabarren, S. E.
,
Adams, G. P.
,
Ratto, Marcelo
,
[Ovarian estradiol modulates the stimulatory effect of ovulation-inducing factor (OIF) on pituitary LH secretion in llamas]
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Ovarian estradiol modulates the stimulatory effect of ovulation-inducing factor (OIF) on pituitary LH secretion in llamas