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We determined if laterality of ovulation and intrauterine embryo location differentially induces changes in the mesometrial/endometrial vascularization area (MEVA) between uterine horns, during and after embryo migration, elongation and implantation in llamas. Adult, non-pregnant and non-lactatin...


Ratto, Marcelo H.Urra, FelipeSilva Jiménez, Mauricio[Laterality of Ovulation and Presence of the Embryo Do Not Affect Uterine Horn Blood Flow During the First Month of Gestation in Llamas]Laterality of Ovulation and Presence of the Embryo Do Not Affect Uterine Horn Blood Flow During the First Month of Gestation in Llamas
Silva Jiménez, MauricioSmulders, Juan P.Guerra, MonserratValderrama, Ximena P.Letelier, ClaudiaAdams, Gregg P.Ratto, Marcelo H.[Cetrorelix suppresses the preovulatory LH surge and ovulation induced by ovulation-inducing factor (OIF) present in llama seminal plasma]Cetrorelix suppresses the preovulatory LH surge and ovulation induced by ovulation-inducing factor (OIF) present in llama seminal plasma

The study was designed to compare the cleavage and blastocysts rate of in vitro matured alpaca and llama oocytes after chemical activation. Alpaca (n=90) and llama (n=85) ovaries were collected at a local slaughterhouse and transported within 2-3 h to the laboratory. Cumulus oocyte complexes (COC...


Ruiz, JaimeLandeo, LeandraMendoza, JoseCorrea, JorgeSilva Jiménez, MauricioRatto, Marcelo H.[In vitro developmental competence of alpaca (Vicugna pacos) and llama (Lama glama) oocytes after parthenogenetic activation]In vitro developmental competence of alpaca (Vicugna pacos) and llama (Lama glama) oocytes after parthenogenetic activation

We evaluated the effect of in vitro maturation time, sperm selection and oxygen tension on alpaca embryo development. In Experiment I, Cumulus Oocyte-Complexes (COCs) were obtained from abattoir ovaries and in vitro matured in TCM-199 for 24 (n = 217), 28 (215), or 32 h (223) at 38.5 degrees C, h...


Ruiz, JaimeSantayana, R. PauloMendoza, M. JoseLandeo, J. LeandraHuaman, ElizabethTicllacuri, FlamelMujica, L. FidelSilva Jiménez, MauricioRatto, Marcelo H.[Effect of oocyte maturation time, sperm selection method and oxygen tension on in vitro embryo development in alpacas]Effect of oocyte maturation time, sperm selection method and oxygen tension on in vitro embryo development in alpacas

The aim of this study was to evaluate the endometrial vascularization area (EVA) of both uterine horns in llamas subjected to different intrauterine treatments resembling physiological conditions after a single mating. Llamas with a growing follicle (>= 8 mm) were randomly assigned to: a) single ...


Urra, FelipeRatto, Marcelo H.Silva Jiménez, Mauricio[Evaluation of the effect of mating, intrauterine deposition of raw seminal plasma or seminal plasma purified beta-NGF on endometrial vascularization in llamas]Evaluation of the effect of mating, intrauterine deposition of raw seminal plasma or seminal plasma purified beta-NGF on endometrial vascularization in llamas
Peralta, Oscar A.Bucher, DanaiFernandez, AnaBerland Olea, MarcoStrobel, PabloRamirez, AlfredoRatto, Marcelo H.Concha, Ilona[Granulocyte-macrophage colony stimulating factor (GM-CSF) enhances cumulus cell expansion in bovine oocytes]Granulocyte-macrophage colony stimulating factor (GM-CSF) enhances cumulus cell expansion in bovine oocytes

Llamas are considered to be reflex ovulators. However, semen from these animals is reported to be rich in ovulation-inducing factor(s), one of which has been identified as nerve growth factor (NGF). These findings suggest that ovulation in llamas may be elicited by chemical signals contained in s...


Berland Olea, MarcoUlloa Leal, CesarBarria, MiguelWright, HollisDissen, Gregory A.Silva Jiménez, MauricioOjeda, Sergio R.Ratto, Marcelo H.[Seminal Plasma Induces Ovulation in Llamas in the Absence of a Copulatory Stimulus: Role of Nerve Growth Factor as an Ovulation-Inducing Factor]Seminal Plasma Induces Ovulation in Llamas in the Absence of a Copulatory Stimulus: Role of Nerve Growth Factor as an Ovulation-Inducing Factor

The ovulation-inducing effect of seminal plasma was first suggested in Bactrian camels over 30 years ago, initiating a long search to identify the 'ovulation-inducing factor' (OIF) present in camelids semen. During the last decade, primarily in llamas and alpacas, this molecule has been intensive...


Silva Jiménez, MauricioPaiva, LuisRatto, Marcelo H.[Ovulation mechanism in South American Camelids: The active role of beta-NGF as the chemical signal eliciting ovulation in llamas and alpacas]Ovulation mechanism in South American Camelids: The active role of beta-NGF as the chemical signal eliciting ovulation in llamas and alpacas

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