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The objective of the present study was to compare the ovarian follicular response, cumulus-oocyte complex (COC) collection rate, and maturational status of COC collected from alpacas subsequent to treatment with two different superstimulatory protocols. Alpacas (n = 7 per group) were treated with...


Ratto Fuster, MarceloGómez, C.Berland Olea, MarcoAdams, G.P.[Effect of ovarian superstimulation on COC collection and maturation in alpacas]Effect of ovarian superstimulation on COC collection and maturation in alpacas

Neospora caninum is a recently recognised parasite that produces infection in dogs and herbivorous. In cows, the disease does not produce clinical signs of illness, instead it produces losses. Chile has a large dairy industry and pregnancy losses in that system seem to be economically important. ...

Los abortos bovinos causan importantes pérdidas económicas a la ganadería mundial. Una parte importante de ellos permanecen aún con etiología desconocida, y de los diagnosticados, la mayoría corresponden a causas infecciosas. Dentro de ellos los abortos ocasionados por protozoos han tomado releva...


Patitucci, A.N.Pérez, M.J.Lüders Post, CarlosRatto Fuster, MarceloDumont, A.G.[Evidencia serológica de infección por Neospora caninum en rebaños lecheros del Sur de Chile][Serologic evidence of Neospora infection in dairy cattle in Chile]Serologic evidence of Neospora infection in dairy cattle in Chile

Se comparó el desarrollo in vitro de ovocitos obtenidos de ovarios de vaca de matadero, madurados, fecundados y cultivados in vitro bajo dos sistemas. Los ovocitos fueron cultivados en un medio de maduración a 39 °C, 5 % de CO2 y humedad relativa de 95 % durante 22 horas. Posteriormente, fuer...

The in vitro development of matured and fertilized bovine oocytes was compared between two culture systems. Oocytes were collected by aspiration of follicles of 3-8 mm in diameter using an 18g needle. After morphological selection the oocytes were incubated at 39 0C, 5 % C02 y 95 % relative humid...


Ratto Fuster, MarceloBerland Olea, MarcoWolter, M.Matamoros, Roberto[Desarrollo de embriones de bovino obtenidos por fecundación in vitro cultivados con células oviductales o medio condicionado y transferidos a hembras receptoras][Bovine embryo development produced by in vitro fertilization cultured with oviductal cell or conditioned medium and transfer to recipients]Bovine embryo development produced by in vitro fertilization cultured with oviductal cell or conditioned medium and transfer to recipients

This study was conducted to determine the use of repeated transvaginal ultrasound-guided cumulus oocyte complex (COC) aspiration on COC recovery rate, in vitro embryo production (IVP) and subsequent pregnancy rates in Holstein Friesian (HF) and Aberdeen Angus (AA) cows (Experiment 1), and in preg...


Ratto Fuster, MarceloPeralta, O.A.Mogollon, G.Strobel, P.Correa, J.[Transvaginal ultrasound-guided cumulus oocyte complexes aspiration and in vitro embryo production in suckled beef and lactating dairy cattle on pasture-based management conditions]Transvaginal ultrasound-guided cumulus oocyte complexes aspiration and in vitro embryo production in suckled beef and lactating dairy cattle on pasture-based management conditions

The objectives of this study were (1) to determine the effect of rabbit seminal plasma on LH secretion and ovulation using the llama animal model as an in vivo ovulation bioassay and (2) to determine the effect of llama or rabbit seminal plasma on ovulation induction in the rabbit model. In Exper...


Silva Jiménez, MauricioNino, A.Guerra, M.Letelier, C.Valderrama, X.P.Adams, G.P.Ratto Fuster, Marcelo[Is an ovulation-inducing factor (OIF) present in the seminal plasma of rabbits?]Is an ovulation-inducing factor (OIF) present in the seminal plasma of rabbits?

This study was designed to determine the effect of location of the preovulatory dominant follicle and stage of ovarian follicle development on ovulation rate and embryo survival in alpacas. In Experiment 1, mature lactating alpacas were randomly assigned to one of two groups according to the loca...


Ratto Fuster, MarceloCervantes, M.Norambuena, C.Silva Jiménez, MauricioMiragaya, M.Huanca, W.[Effect of location and stage of development of dominant follicle on ovulation and embryo survival rate in alpacas]Effect of location and stage of development of dominant follicle on ovulation and embryo survival rate in alpacas

The objective of the study was to compare the ovulatory response and embryo production in llamas (Lama glama) treated with a single dose of equine chorionic gonadotropin (eCG) alone or combined with intravaginal medroxyprogesterone acetate (MPA) at the time of follicular wave emergence. Llamas wi...


Huanca, W.Cordero, A.Huanca, T.Cárdenas, O.Adams, G.P.Ratto Fuster, Marcelo[Ovarian response and embryo production in llamas treated with equine chorionic gonadotropin alone or with a progestin-releasing vaginal sponge at the time of follicular wave emergence]Ovarian response and embryo production in llamas treated with equine chorionic gonadotropin alone or with a progestin-releasing vaginal sponge at the time of follicular wave emergence

The objective was to evaluate the developmental competence of cumulus-oocyte complexes (COC) collected by follicular aspiration in llamas treated with FSH or eCG. Llamas were assigned randomly to two groups (n = 16 per group) and treated, at the time of ovarian follicular wave emergence, with eit...


Berland Olea, MarcoBaer, A.Ruíz, J.Parraguez, VíctorMorales, P.Adams, G.P.Ratto Fuster, Marcelo[In vitro fertilization and development of cumulus oocytes complexes collected by ultrasound-guided follicle aspiration in superstimulated llamas]In vitro fertilization and development of cumulus oocytes complexes collected by ultrasound-guided follicle aspiration in superstimulated llamas

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