Transgenic banana (Musa acuminata 'Gros Michel') integrating either of two rice chitinase genes was generated and its resistance to Black Leaf Streak disease caused by the fungus Mycosphaerella fijiensis was tested using a leaf disk bioassay. PCR screening indicated the presence of the hpt select...
Kovacs, G.
,
Sagi, L.
,
Jacon, G.
,
Arinaitwe, G.
,
Busogoro, J.P.
,
Thiry, E.
,
Strosse, H.
,
Swennen, R.L.
,
Remy, S.
,
[Expression of a rice chitinase gene in transgenic banana (''Gros Michel'', AAA genome group) confers resistance to black leaf streak disease]
,
Expression of a rice chitinase gene in transgenic banana (''Gros Michel'', AAA genome group) confers resistance to black leaf streak disease
Swennen, R.L.
,
Carpentier, S.C.
,
Henry, I.M.
,
Vertommen, A.
,
Houwe, I. van den
,
Kovacs, G.
,
Sagi, L.
,
Remy, S.
,
Panis, B.
,
[From fundamental research discoveries to applications for banana improvement]
,
From fundamental research discoveries to applications for banana improvement
Gene expression analysis by reverse transcriptase real-time or quantitative polymerase chain reaction (RT-qPCR) is becoming widely used for nonmodel plant species. Given the high sensitivity of this method, normalization using multiple housekeeping or reference genes is critical, and careful sele...
Podevin, N.
,
Krauss, A.
,
Henry, I.
,
Swennen, R.L.
,
Remy, S.
,
[Selection and validation of reference genes for quantitative RT-PCR expression studies of the non-model crop Musa]
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Selection and validation of reference genes for quantitative RT-PCR expression studies of the non-model crop Musa
In order to introduce currently-available genes with agronomical value into banana, two genetic transformation protocols have been optimized. Firstly, regenerable protoplasts isolated from embryogenic cell suspensions of the cultivar Bluggoe have been used for the introduction of several chimaeri...
Sagi, L.
,
Remy, S.
,
Verelst, B.
,
Panis, B.
,
Cammue, B.P.
,
Volckaert, G.
,
Swennen, R.
,
[Transient gene expression in transformed banana (Musa cv. Bluggoe) protoplasts and embryogenic cell suspensions]
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Transient gene expression in transformed banana (Musa cv. Bluggoe) protoplasts and embryogenic cell suspensions
The term banana is meant throughout this chapter to cover dessert and cooking bananas as well as plantains. With an estimated total production of more than 76 million tons in 1992 (FAO 1993), banana is the no. 1 fruit crop in the world. Ninety-nine percent of world's annual production is cultivat...
Sagi, L.
,
Remy, S.
,
Verelst, B.
,
Swennen, R.
,
Panis, B.
,
[Genetic transformation in Musa species (Banana)]
,
Genetic transformation in Musa species (Banana)
We have developed a simple protocol to allow the production of transgenic banana plants. Foreign genes were delivered into embryogenic suspension cells using accelerated particles coated with DNA. Bombardment parameters were optimized for a modified particle gun resulting in high levels of transi...
Sági, L.
,
Panis, B.
,
Remy, S.
,
Schoofs, H.
,
Smet, K. de
,
Swennen, R.
,
Cammue, B.P.
,
[Genetic transformation of banana and plantain (Musa spp.) via particle bombardment]
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Genetic transformation of banana and plantain (Musa spp.) via particle bombardment
Electroporation conditions were established for transient expression of introduced DNA in banana (Musa spp., cv. 'Bluggoe') protoplasts isolated from regenerable embryogenic cell suspensions. The following parameters were found to be highly influential: electroporation buffer, polyethylene glycol...
In plants, RNA- based gene silencing mediated by small RNAs functions at the transcriptional or post-transcriptional level to negatively regulate target genes, repetitive sequences, viral RNAs and/or transposon elements. Post-transcriptional gene silencing (PTGS) or the RNA interference (RNAi) ap...
Dang Thi Tuong Vi
,
Windelinckx, S.
,
Henry, I.
,
Coninck, B. de
,
Cammue, B.P.A.
,
Swennen, R.L.
,
Remy, S.
,
[Assessment of RNAi-induced silencing in banana (Musa spp.)]
,
Assessment of RNAi-induced silencing in banana (Musa spp.)