We have developed a simple protocol to allow the production of transgenic banana plants. Foreign genes were delivered into embryogenic suspension cells using accelerated particles coated with DNA. Bombardment parameters were optimized for a modified particle gun resulting in high levels of transi...
Sági, L.
,
Panis, B.
,
Remy, S.
,
Schoofs, H.
,
Smet, K. de
,
Swennen, R.
,
Cammue, B.P.
,
[Genetic transformation of banana and plantain (Musa spp.) via particle bombardment]
,
Genetic transformation of banana and plantain (Musa spp.) via particle bombardment
Electroporation conditions were established for transient expression of introduced DNA in banana (Musa spp., cv. 'Bluggoe') protoplasts isolated from regenerable embryogenic cell suspensions. The following parameters were found to be highly influential: electroporation buffer, polyethylene glycol...
In plants, RNA- based gene silencing mediated by small RNAs functions at the transcriptional or post-transcriptional level to negatively regulate target genes, repetitive sequences, viral RNAs and/or transposon elements. Post-transcriptional gene silencing (PTGS) or the RNA interference (RNAi) ap...
Dang Thi Tuong Vi
,
Windelinckx, S.
,
Henry, I.
,
Coninck, B. de
,
Cammue, B.P.A.
,
Swennen, R.L.
,
Remy, S.
,
[Assessment of RNAi-induced silencing in banana (Musa spp.)]
,
Assessment of RNAi-induced silencing in banana (Musa spp.)