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The aim of the present investigations was to test a novel technology comprising cryoprotectant-free vitrification of the spermatozoa of rainbow trout and to study the ability of sucrose and components of seminal plasma to protect these cells from cryoinjuries. Spermatozoa were isolated and vitrif...


Merino, O.Risopatrón, J.Sánchez, R.Isachenko, E.Figueroa Villalobos, ElíasValdebenito Isler, IvánIsachenko, V.[Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness]Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness

The knowledge of sperm quality in the broodstock males of different ages is a prerequisite to identify the reproductive ability of cultivated fish for the hatchery management. Thus, in this work, we analysed sperm function of the semen stored of broodstock males of rainbow trout (Oncorhychus myki...


Risopatrón, J.Merino, O.Cheuqueman, C.Figueroa, E.Sánchez, R.Farías, Jorge G.Valdebenito, I.[Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)]Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)

Sperm motility in fish with external fertilization is critical for reproductive efficiency in aquaculture, especially in salmonids. Gamete preservation techniques, such as cryopreservation, however, reduce sperm motility and fertilizing capacity. Very few studies have addressed cryodamage from en...


Lee Estévez, M.Herrera, L.Díaz, R.Beltrán, J.Figueroa Villalobos, ElíasDumorné, K.Ulloa Rodríguez, P.Short, S.Risopatrón, J.Valdebenito Isler, IvánFarías, J.[Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility]Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility

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