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Risopatron, J.Sanchez, R.Sepulveda, N.Villagran, E.Pena, P.[Seleccion de espermatozoides de bovino desde semen congelado-descongelado: comparacion de dos metodos]Seleccion de espermatozoides de bovino desde semen congelado-descongelado: comparacion de dos metodos

The objective of this study was to determine the effect of freezing on the function in Atlantic salmon Salmo salar spermatozoa. The semen was frozen in Cortland's medium+1.3M dimethyl sulphoxide+0.3M glucose+2% bovine serum albumin (final concentration) in a ratio of 1:3 (semen:cryoprotectant) as...


Figueroa Villalobos, ElíasValdebenito Isler, IvánMerino, O.Ubilla Madrid, AndreaRisopatron, J.Farias, J. G.[Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology]Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology

The knowledge of sperm quality in the broodstock males of different ages is a prerequisite to identify the reproductive ability of cultivated fish for the hatchery management. Thus, in this work, we analysed sperm function of the semen stored of broodstock males of rainbow trout (Oncorhychus myki...


Risopatron, J.Merino, O.Cheuqueman, C.Figueroa Villalobos, ElíasSanchez, R.Farias, J. G.Valdebenito Isler, Iván[Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)]Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)

The use of α-tocopherol and ascorbic acid in freezing medium prevents lipoperoxidation and damage by ROS (reactive oxygen species); however, the effects of these antioxidants in salmonid spermatozoa have not been studied. In this work the protective effects of antioxidants on the enzymatic activi...


Figueroa Villalobos, ElíasFarias, J.G.Lee Estevez, M.Valdebenito Isler, IvánRisopatron, J.Magnotti, C.Romero, J.Watanabe, I.Oliveira, R.P.S.[Sperm cryopreservation with supplementation of a-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)]Sperm cryopreservation with supplementation of a-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)

In vitro storage of salmonid eggs leads to aging of the cells causing a decline in quality and reducing their capacity to develop and produce embryos. The quality of salmonid embryos is assessed by morphologic analyses; however, data on the application of biomarkers to determine the cell viabilit...


Ubilla Madrid, AndreaValdebenito Isler, IvánArias, M. E.Risopatron, J.[Viability and DNA fragmentation of rainbow trout embryos (Oncorhynchus mykiss) obtained from eggs stored at 4 degrees C]Viability and DNA fragmentation of rainbow trout embryos (Oncorhynchus mykiss) obtained from eggs stored at 4 degrees C

This study was designed to test a vitrification method on spermatozoa of sex-reversed rainbow trout and determine the ability of sucrose, dimethyl sulfoxide (DMSO), bovine serum albumin (BSA) and seminal plasma (SP) to protect these cells from cryoinjuries. The vitrification medium comprised a st...


Figueroa Villalobos, ElíasRisopatron, J.Sanchez, R.Isachenko, E.Merino, O.Isachenko, V.Valdebenito Isler, Iván[Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters]Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters

The aims of this investigation were to test a novel technology comprising cryoprotectant-free vitrification of the spermatozoa of rainbow trout and to study the ability of sucrose and components of seminal plasma to protect these cells from cryo-injuries. Spermatozoa were isolated and vitrified u...


Merino, O.Sanchez, R.Risopatron, J.Isachenko, E.Katkov, I. I.Figueroa Villalobos, ElíasValdebenito Isler, IvánMallmann, P.Isachenko, V.[Cryoprotectant-free vitrification of fish (Oncorhynchus mykiss) spermatozoa: first report]Cryoprotectant-free vitrification of fish (Oncorhynchus mykiss) spermatozoa: first report

Spermatological research of the Patagonian blennie was carried, specifically biometric parameters, sperm density, sperm count and motility in different activation mediums (815, 716, 590 and 0mOSmkg(-1)), at different temperatures (5, 10 and 15 degrees C) and pH levels (5, 7 and 9). The results in...


Valdebenito Isler, IvánCosson, J.Contreras, P.Sanchez, J. C.Oliveira, R. P. S.Risopatron, J.Farias, J. G.Figueroa Villalobos, Elías[Spermatological research of experimentally farmed Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopsidae) in Chile]Spermatological research of experimentally farmed Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopsidae) in Chile

The aim of the present investigations was to test a novel technology comprising cryoprotectant-free vitrification of the spermatozoa of rainbow trout and to study the ability of sucrose and components of seminal plasma to protect these cells from cryoinjuries. Spermatozoa were isolated and vitrif...


Merino, O.Risopatron, J.Sanchez, R.Isachenko, E.Figueroa Villalobos, ElíasValdebenito Isler, IvánIsachenko, V.[Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness]Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness
Figueroa Villalobos, ElíasLee Estevez, M.Risopatron, J.Valdebenito Isler, IvánPinheiro de Souza, R.Farias, J. G.[EFFECTS OF CRYOPRESERVATION ON OXIDATIVE STRESS AND ANTIOXIDANT ENZYME ACTIVITY IN SPERMATOZOA OF ATLANTIC SALMON (Salmo salar)]EFFECTS OF CRYOPRESERVATION ON OXIDATIVE STRESS AND ANTIOXIDANT ENZYME ACTIVITY IN SPERMATOZOA OF ATLANTIC SALMON (Salmo salar)

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