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The use of alpha-tocopherol and ascorbic acid in freezing medium prevents lipoperoxidation and damage by ROS (reactive oxygen species); however, the effects of these antioxidants in salmonid spermatozoa have not been studied. In this work the protective effects of antioxidants on the enzymatic ac...


Figueroa Villalobos, ElíasFarias, J. G.Lee Estevez, M.Valdebenito Isler, IvánRisopatron, J.Magnotti, C.Romero, J.Watanabe, IOliveira, R. P. S.[Sperm cryopreservation with supplementation of alpha-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)]Sperm cryopreservation with supplementation of alpha-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)

Short-term storage of semen is a useful strategy for preservation of fish spermatozoa in reproductive management, however, no information is available on Patagonian blenny (Eleginops maclovinus) semen. This study was designed to evaluate the effects of in vitro storage on sperm function parameter...


Contreras, P.Ulloa, P.Merino, O.Valdebenito Isler, IvánFigueroa Villalobos, ElíasFarias, J.Risopatron, J.[Effect of short-term storage on sperm function in Patagonian blenny (Eleginops maclovinus) sperm]Effect of short-term storage on sperm function in Patagonian blenny (Eleginops maclovinus) sperm

The short-term storage of salmonid semen is a viable method for in vitro fertilisation. Previous studies have found that short-term storage affects sperm motility, compromising quality and fertilising capacity. However, the functional characteristics of the spermatozoa of O.mykiss during storage ...


Trigo, P.Merino, O.Figueroa Villalobos, ElíasValdebenito Isler, IvánSanchez, R.Risopatron, J.[Effect of short-term semen storage in salmon (Oncorhynchus mykiss) on sperm functional parameters evaluated by flow cytometry]Effect of short-term semen storage in salmon (Oncorhynchus mykiss) on sperm functional parameters evaluated by flow cytometry
Ulloa, P.Contreras, P.Dumorne, K.Lee-Estevez, M.Figueroa Villalobos, ElíasValdebenito Isler, IvánRisopatron, J.Farias, J. G.[Patagonian blenny (Eleginops maclovinus) spermatozoa characterization and quality markers evaluation under chilling storage.]Patagonian blenny (Eleginops maclovinus) spermatozoa characterization and quality markers evaluation under chilling storage.

Short-term storage of semen is a useful strategy for preservation of fish spermatozoa. However, there is a significantly decrease on sperm function mainly due to oxidative stress. In this way, sodium alginate plays an important role as free radical scavenger compound. Accordingly, the aim of our ...


Merino, O.Figueroa Villalobos, ElíasCheuqueman, C.Valdebenito Isler, IvánIsachenko, V.Isachenko, E.Sanchez, R.Farias, J.Risopatron, J.[Short-term storage of salmonids semen in a sodium alginate-based extender]Short-term storage of salmonids semen in a sodium alginate-based extender

This study was designed to test a vitrification method in Atlantic salmon spermatozoa and determine the capacity of seminal plasma (SP) to protect these cells from cryoinjuries. The vitrification medium consisted of a standard buffer for fish spermatozoa (Cortland medium) + 10% DMSO + 2% BSA + 0....


Figueroa Villalobos, ElíasMerino, O.Risopatron, J.Isachenko, V.Sanchez, R.Effer Roldan, BrianIsachenko, E.Farias, J. G.Valdebenito Isler, Iván[Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification]Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification

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