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Guidelines are provided to acquaint the personnel of national programs and other collaborators with the procedures to recover, propagate, and transplant culture materials after their arrival. These include: (1) recovery of the cultures by direct potting of cassava plants after short trips; 1 wk. ...


Roca, William M.Rodríguez, J.A.Mafla, G.Roa E, J.C.[Procedures for recovering cassava clones distributed in vitro]Procedures for recovering cassava clones distributed in vitro

Plant tissue culture includes a wide range of techniques: protoplast, cell, anther, callus, meristem and organ culture, all having one common factor: the growth of vegetative material in an aseptic environment; i,e., the sterilized culture medium in test tubes. In spite of the fact that several o...


Roca, William M.[Cultivo de tejidos en yuca]Cultivo de tejidos en yuca

Methods of vegetative reproduction are discussed using the results obtained with cassava and potato. Results of clone cleaning by heat therapy and in vitro meristem culture are given. Two methods for rapid cassava multiplication are proposed: by sprouts from cuttings and single leaf cuttings, whi...


Roca, William M.[Reproduccion vegetativa de materiales para la siembra libres de enfermedades]Reproduccion vegetativa de materiales para la siembra libres de enfermedades
Roca, William M.[Meristem culture in cassava: principles and methodology: principles and procedures]Meristem culture in cassava: principles and methodology: principles and procedures

Several techniques used to eliminate viruses from cassava are described, namely heat therapy, apical tissue culture, and micropropagation. Virological tests to index cassava viruses are also indicated as follows: ELISA, dsRNA, mechanical inoculation of leaf sap in plants on Chenopodium quinoa, an...


Roca, William M.Nolt, Barry L.Mafla, G.Roa E, J.C.Reyes, R.[Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz)]Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz)

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