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Restriction fragment analysis was used to determine the variation of chloroplast DNA (cpDNA) among and within different species of Passiflora. Total DNAs of 35 accessions, representing 12 species, were digested with four restriction endonucleases. Southern blots of the restriction digests were th...
Los trabajos realizados en varias especies de plantas revelan que una porción significativa de los genes que se expresan en el esporofito también lo hacen en el grano de polen, en consecuencia la presión de selección a nivel de polen debe conducir a modificaciones en caracteres cualitativos o cu...
The lack of good quality planting material of farmers' cassava varieties, produced locally and at low cost, is a major constraint limiting the expansion of cassava production in Colombia. This article describes the adaptation of conventional cassava propagation to a low-input scheme for rural tis...
Transgenic plants of cassava (Manihot esculenta) resistant to the herbicide Basta were obtained through Agrobacterium-mediated transformation. The plants also expressed the uidA gene and two were positive for PCR- and/or Southern-based detection of the nptII gene. Somatic-embryo-derived cotyledon...
Estado actual del mejoramiento del arroz mediante la utilización de especies silvestres de arroz en CIAT. Este trabajo presenta el uso de especies silvestres de arroz en la obtención de germoplasma mejorado con mayor potencial de rendimiento y una base genética ampliada. Tres especies silvestres ...
Se construyó una pequeña librería de cDNA de fríjol (phaseolus vulgaris L.) de alrededor de 1500 clones, con el fin de incrementar la saturación del mapa de ligamiento de fríjol con marcadores de RFLPs. Para la generación de la librería se utilizó la técnica de amplificación por PCR (Jepson et al...
Genetic analysis based on Random Amplified Polymorphic DNA (RAPD) was carried out on 52 accessions representing 14 species of the genus Passiflora L. using 50 random 10-mer primers. A dendrogram constructed using the Dice similarity coefficient and the UPGMA algorithm based on 626 reproducible po...
Breeding goals of yield increases, root quality improvement, and disease resistance in cassava are considerably slowed down by biological characteristics of the crop, which includes a long growth cycle, a heterozygous genetic background and a poor knowledge of the organization of crop diversity. ...
A polyacrylamide gel electrophoresis procedure was developed for discriminating cultivars of the forage legume Pueraria phaseoloides on the basis of the patterns of cotyledon proteins. The genotypic marker proteins were extracted with 5M acetic acid and electrophoresed at pH 3.1 in aluminum lacta...
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