A genetic linkage map of cassava has been constructed with 132 RFLPs, 30 RAPDs, 3 microsatellites, and 3 isoenzyme markers segregating from the heterozygous female parent of an intraspecific cross. The F1 cross was made between ‘TMS 30572’ and ‘CM?2177-2’, elite cassava cultivars from Nigeria and...
Fregene, Martin A.
,
Angel, F.
,
Gómez, R.
,
Rodríguez, F.
,
Chavarriaga Aguirre, Paul
,
Roca, William M.
,
Tohme, Joseph M.
,
Bonierbale, Merideth W.
,
[A molecular genetic map of cassava (Manihot esculenta Crantz)]
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A molecular genetic map of cassava (Manihot esculenta Crantz)
Hoyos, R.A.
,
Roca, William M.
,
Hosfield, GL
,
Mayer, Jorge Edgard
,
[Particle gun transient expression of sexual embryos of common bean]
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Particle gun transient expression of sexual embryos of common bean
Restriction fragment analysis was used to determine the variation of chloroplast DNA (cpDNA) among and within different species of Passiflora. Total DNAs of 35 accessions, representing 12 species, were digested with four restriction endonucleases. Southern blots of the restriction digests were th...
Sánchez, I.
,
Angel, F.
,
Grum, M.
,
Duque E., Myriam Cristina
,
Lobo Arias, M
,
Tohme, Joseph M.
,
Roca, William M.
,
[Variability of chloroplast DNA in the genus Passiflora L.]
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Variability of chloroplast DNA in the genus Passiflora L.
Se construyó una pequeña librería de cDNA de fríjol (phaseolus vulgaris L.) de alrededor de 1500 clones, con el fin de incrementar la saturación del mapa de ligamiento de fríjol con marcadores de RFLPs. Para la generación de la librería se utilizó la técnica de amplificación por PCR (Jepson et al...
Ramírez, H.
,
Mayer, Jorge Edgard
,
Roca, William M.
,
Tohme, Joseph M.
,
López, Y.
,
[Construcción de una genoteca de cDNA de fríjol (Phaseolus vulgaris L.) para mapeo genético]
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Construcción de una genoteca de cDNA de fríjol (Phaseolus vulgaris L.) para mapeo genético
Several important aspects of the use of molecular markers in cassava germplasm characterization and in plant breeding programs are indicated. Basic concepts considered include protein and DNA electrophoresis. In the case of cassava, isoenzyme electrophoresis was applied to confirm duplicates dete...
Ramírez, H.
,
Calderón, A.
,
Roca, William M.
,
[Tecnicas moleculares para evaluar y mejorar el germoplasma vegetal]
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Tecnicas moleculares para evaluar y mejorar el germoplasma vegetal
Angel, F.
,
Arias Garzón, D.I.
,
Roca, William M.
,
[DNA polymorphism and DNA fingerprinting of cassava : final report collaborative project CIAT-IBPGR]
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DNA polymorphism and DNA fingerprinting of cassava : final report collaborative project CIAT-IBPGR